Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism

The Plant Cell
Alexandra ChanocaMarisa S Otegui

Abstract

Anthocyanins are flavonoid pigments synthesized in the cytoplasm and stored inside vacuoles. Many plant species accumulate densely packed, 3- to 10-μm diameter anthocyanin deposits called anthocyanin vacuolar inclusions (AVIs). Despite their conspicuousness and importance in organ coloration, the origin and nature of AVIs have remained controversial for decades. We analyzed AVI formation in cotyledons of different Arabidopsis thaliana genotypes grown under anthocyanin inductive conditions and in purple petals of lisianthus (Eustoma grandiorum). We found that cytoplasmic anthocyanin aggregates in close contact with the vacuolar surface are directly engulfed by the vacuolar membrane in a process reminiscent of microautophagy. The engulfed anthocyanin aggregates are surrounded by a single membrane derived from the tonoplast and eventually become free in the vacuolar lumen like an autophagic body. Neither endosomal/prevacuolar trafficking nor the autophagy ATG5 protein is involved in the formation of AVIs. In Arabidopsis, formation of AVIs is promoted by both an increase in cyanidin 3-O-glucoside derivatives and by depletion of the glutathione S-transferase TT19. We hypothesize that this novel microautophagy mechanism also mediates...Continue Reading

Citations

Feb 26, 2016·Frontiers in Plant Science·Valentina PasseriFrancesca M Quattrocchio
Mar 5, 2016·Bioscience, Biotechnology, and Biochemistry·Nobukazu Shitan
Aug 9, 2016·The Plant Journal : for Cell and Molecular Biology·Alexandra ChanocaMarisa S Otegui
Feb 24, 2018·Journal of Experimental Botany·Tamar Avin-WittenbergHenri Batoko
Jan 10, 2018·Cells·Jessica MarionBéatrice Satiat-Jeunemaitre
Mar 22, 2018·Annual Review of Plant Biology·Tomoo ShimadaIkuko Hara-Nishimura
Mar 15, 2018·Annual Review of Plant Biology·Richard S Marshall, Richard D Vierstra
Aug 25, 2017·Autophagy·Victoria Sanchez-VeraEva Sundberg
Jul 13, 2018·Plant, Cell & Environment·Tamar Avin-Wittenberg
Sep 20, 2017·Journal of Experimental Botany·Takayuki TohgeAlisdair R Fernie
Aug 26, 2017·The Journal of Cell Biology·Masahide OkuYasuyoshi Sakai
Oct 17, 2018·Natural Product Reports·Camilla KnudsenTomas Laursen
Oct 30, 2015·Nature·Diane C Bassham
Jun 10, 2017·The EMBO Journal·Lorenzo GalluzziGuido Kroemer
Nov 16, 2019·Cells·Qinwu ChenCéline Masclaux-Daubresse
Apr 9, 2020·Cells·Katarzyna SieńkoShino Goto-Yamada
Nov 23, 2017·Frontiers in Plant Science·Alon Cna'aniAlexander Vainstein
May 1, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Masahide Oku, Yasuyoshi Sakai
Aug 28, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Laura A ChathamJohn A Juvik
Sep 11, 2020·Journal of Cell Science·Sebastian Schuck
Oct 27, 2017·Journal of Experimental Botany·Tamara PecenkováViktor Žárský
Aug 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qianqian ShiYanlong Zhang
Dec 7, 2018·Open Biology·Jie Tang, Diane C Bassham
Dec 19, 2019·Frontiers in Plant Science·Shino Goto-YamadaKenji Yamada
Dec 24, 2018·Plant Signaling & Behavior·Sakuya Nakamura, Masanori Izumi
Mar 27, 2020·International Journal of Molecular Sciences·Szymon StefaniakSławomir Borek
Aug 23, 2020·Plants·Yifan LiQingjun Xie
Nov 3, 2017·Journal of Experimental Botany·Gian Pietro Di SansebastianoCarine de Marcos Lousa
Jul 30, 2018·Plant Reproduction·Edith FrancozHelen M North
Jul 28, 2017·Frontiers in Plant Science·Quanquan YanWenli Chen
Nov 23, 2020·The Plant Journal : for Cell and Molecular Biology·Germán RobertKohki Yoshimoto
Feb 13, 2021·Sensors·Jenu V ChackoKevin W Eliceiri
Feb 16, 2021·G3 : Genes - Genomes - Genetics·Laura A Chatham, John A Juvik
Jul 19, 2017·Plant Science : an International Journal of Experimental Plant Biology·Diane C Bassham, Gustavo C MacIntosh
Feb 27, 2018·Phytochemistry·Craig A Schenck, Hiroshi A Maeda
Sep 30, 2018·Current Opinion in Plant Biology·Xinxin DingMarisa S Otegui
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel OliveiraThomas Andlid

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATG proteins

The discovery of autophagy-related ('ATG') proteins in the 1990s greatly advanced the mechanistic understanding of autophagy and clarified the fact that autophagy serves important roles in various biological processes.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms