Anthranilate synthetase, an enzyme specified by the tryptophan operon of Escherichia coli: purification and characterization of component I.

Journal of Bacteriology
J ItoC Yanofsky

Abstract

A procedure employed in the purification of anthranilate synthetase component I of Escherichia coli is described. The purified component appears homogeneous by starch gel electrophoresis and by sedimentation analysis. A molecular weight of 60,000 was estimated by gel filtration of Sephadex G-100. This value is consistent with the molecular weight estimated from the sedimentation and diffusion coefficients. Purified anthranilate synthetase component I cannot use glutamine as substrate and thus has no activity in the reaction of chorismate + l-glutamine --> anthranilate; however, it is active when ammonium sulfate is provided as amino donor. Sucrose density gradient analyses showed that ammonium sulfate does not affect the sedimentation velocity of component I. The ultraviolet absorption and fluorescence spectra of the purified component indicated that it contains tryptophan. Peptide pattern and extract complementation evidence suggested that the protein is a single polypeptide chain. Enzyme activity measurements indicated that wild-type E. coli produces equimolar amounts of at least four of the five polypeptides specified by the operon. Purified anthranilate synthetase component I is inhibited by l-tryptophan.

References

Nov 14, 1966·Journal of Molecular Biology·R G MartinH J Whitfield
Apr 1, 1966·Journal of Bacteriology·E W Nester, R A Jensen
Oct 28, 1966·Journal of Molecular Biology·M E GoldbergC Yanofsky
Nov 14, 1966·Journal of Molecular Biology·C Yanofsky, J Ito
Sep 10, 1962·Biochimica Et Biophysica Acta·D R HELINSKI, C YANOFSKY
Apr 1, 1964·Journal of Molecular Biology·R L SOMERVILLE, C YANOFSKY
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·I P CRAWFORD, J ITO
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX
Jul 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·C LEVINTHALK FETHEROLF
Jan 1, 1946·The Biochemical Journal·T W Goodwin, R A Morton

❮ Previous
Next ❯

Citations

Sep 8, 1975·Molecular & General Genetics : MGG·R Benne, P H Pouwels
Jan 1, 1975·Molecular & General Genetics : MGG·P H Pouwels, J van Rotterdam
Nov 24, 1976·Molecular & General Genetics : MGG·A Moir, W J Brammar
Dec 7, 2013·Bulletin of Mathematical Biology·Emanuel Salazar-Cavazos, Moisés Santillán
Oct 1, 1972·Journal of Bacteriology·E N Baskerville, R Twarog
Feb 1, 1970·Journal of Bacteriology·A Catena, R D DeMoss
Sep 15, 1979·Journal of Molecular Biology·D F Ward, N E Murray
Apr 5, 1974·Journal of Molecular Biology·N Shimizu, M Hayashi
Feb 1, 1988·Journal of Bacteriology·C M Ross, M E Winkler
Nov 15, 1976·Journal of Molecular Biology·A S HopkinsW J Brammar
Mar 19, 1976·Science·N E DixonB Zerner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.