Anti- Versus Pro-Inflammatory Metabololipidome Upon Cupping Treatment

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Qi ZhangYongsheng Li

Abstract

This study aimed to explore the metabololipidome in mice upon cupping treatment. A nude mouse model mimicking the cupping treatment in humans was established by administrating four cupping sets on the back skin for 15 minutes. UPLC-MS/ MS was performed to determine the PUFA metabolome in mice skin and blood before and after cupping treatment. The significantly changed lipids were administered in macrophages to assess the production of pro-inflammatory cytokines IL-6 and TNF-α by ELISA. The anti-inflammatory lipids, e.g. PGE1, 5,6-EET, 14,15-EET, 10S,17S-DiHDoHE, 17R-RvD1, RvD5 and 14S-HDoHE were significantly increased while pro-inflammatory lipids, e.g. 12-HETE and TXB2 were deceased in the skin or plasma post cupping treatment. Cupping treatment reversed the LPS-stimulated IL-6 and TNF-α expression in mouse peritoneal exudates. Moreover, 5,6-EET, PGE1 decreased the level of TNF-α, while 5,6-EET, 5,6-DHET downregulated IL-6 production in macrophages. Importantly, 14,15-EET and 14S-HDoHE inhibited both IL-6 and TNF-α induced by lipopolysaccharide (LPS). 17-RvD1, RvD5 and PGE1 significantly reduced the LPS-initiated TNF-α, while TXB2 and 12-HETE further upregulated the LPS-enhanced IL-6 and TNF-α expression in macrophages. Our r...Continue Reading

Citations

Jul 27, 2018·Annals of the Rheumatic Diseases·Shengwei JinJianguang Wang
Nov 5, 2019·Frontiers in Pharmacology·Tabea Osthues, Marco Sisignano
May 8, 2020·Molecular Medicine·Qi ZhangYongsheng Li
Jan 12, 2021·Journal of Agricultural and Food Chemistry·Zongyuan WuHong Chen
Aug 4, 2021·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Guangrong YangJianguo Sun

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