Anti-activin A antibody (IgY) specifically neutralizes various activin A activities

Proceedings of the Society for Experimental Biology and Medicine
T MurataK Torii

Abstract

Activin A (beta A beta A), originally isolated from ovarian follicular fluids as a follicule-stimulating hormone (FSH) secretion stimulator, has also been identified as an erythroid differentiation factor (EDF), a neuron survival factor and a mesoderm-inducing factor. Thus, activin A is a multifunctional factor, and further studies on its physiological function are important. However, it is very difficult to produce a specific antibody to neutralize the activity of activin A because of its highly conserved amino acid sequence across mammalian species. In this study, we succeeded in generating an antibody against activin A, which can neutralize several activities of activin A, such as the stimulation of FSH secretion from pituitary cells and the induction of the differentiation of erythrocytes in vitro. This antibody did not affect the activity of activin B (beta B beta B), which induces the differentiation of erythrocytes in vitro, and the activity of inhibin A (alpha beta A), which inhibits FSH secretion from pituitary in vitro, but slightly neutralized that of activin AB (beta A beta B). Western blotting analysis showed that this antibody recognized both dimeric and monomeric forms of the beta A subunit of activin and inhibin...Continue Reading

Citations

Oct 19, 2002·Molecular and Cellular Endocrinology·Stacey C ChapmanTeresa K Woodruff
Nov 7, 2012·Expert Opinion on Investigational Drugs·Scott Z FieldsEvangelos Terpos
Mar 1, 2005·Trends in Endocrinology and Metabolism : TEM·Craig A HarrisonDavid M Robertson
Apr 24, 2016·Microscopy·Shelley Mettler IzquierdoWilliam Harriman
Sep 24, 2004·Biology of Reproduction·Sheila A Sweeney, Patricia A Johnson
Dec 13, 2018·Physiological Reviews·Enrrico BloiseFernando M Reis
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G CamenischM Gassmann
Sep 10, 2014·Diabetes, Obesity & Metabolism·T TsurugizawaK Torii
Nov 24, 1999·The Journal of Veterinary Medical Science·Y NishinoM Hara

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