Anti-adaptors provide multiple modes for regulation of the RssB adaptor protein

Genes & Development
Aurelia BattestiS Gottesman

Abstract

RpoS, an RNA polymerase σ factor, controls the response of Escherichia coli and related bacteria to multiple stress responses. During nonstress conditions, RpoS is rapidly degraded by ClpXP, mediated by the adaptor protein RssB, a member of the response regulator family. In response to stress, RpoS degradation ceases. Small anti-adaptor proteins--IraP, IraM, and IraD, each made under a different stress condition--block RpoS degradation. RssB mutants resistant to either IraP or IraM were isolated and analyzed in vivo and in vitro. Each of the anti-adaptors is unique in its interaction with RssB and sensitivity to RssB mutants. One class of mutants defined an RssB N-terminal region close to the phosphorylation site and critical for interaction with IraP but unnecessary for IraM and IraD function. A second class, in the RssB C-terminal PP2C-like domain, led to activation of RssB function. These mutants allowed the response regulator to act in the absence of phosphorylation but did not abolish interaction with anti-adaptors. This class of mutants is broadly resistant to the anti-adaptors and bears similarity to constitutively activated mutants found in a very different PP2C protein. The mutants provide insight into how the anti-ada...Continue Reading

References

Jan 22, 1998·Applied and Environmental Microbiology·P R Jensen, K Hammer
Mar 12, 1998·Journal of Bacteriology·Y Zhou, S Gottesman
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·S K AmesL J Kenney
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SchusterR B Bourret
Apr 8, 2006·Genes & Development·Alexandre BougdourSusan Gottesman
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Houra MerrikhSusan T Lovett
Jul 7, 2009·Annual Review of Microbiology·Rong Gao, Ann M Stock
Oct 13, 2009·Journal of Bacteriology·Houra MerrikhSusan T Lovett
Jan 19, 2010·Current Opinion in Microbiology·Rong Gao, Ann M Stock
Mar 10, 2010·Current Opinion in Microbiology·Robert B Bourret
Mar 17, 2010·Current Opinion in Microbiology·Michael Y Galperin
Jun 7, 2011·Annual Review of Microbiology·Aurelia BattestiSusan Gottesman
Jul 31, 2012·Methods : a Companion to Methods in Enzymology·Aurélia Battesti, Emmanuelle Bouveret

❮ Previous
Next ❯

Citations

Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Stephen C SmithKathleen R Ryan
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Aurelia BattestiSusan Gottesman
Oct 10, 2015·Cell·Kamal Kishore JoshiPeter Chien
May 23, 2014·Current Biology : CB·Monica S Guo, Carol A Gross
May 4, 2016·Nucleic Acids Research·Hyun-Jung Lee, Susan Gottesman
Aug 12, 2016·Cell Host & Microbe·Ferric C FangAndrés Vázquez-Torres
Nov 29, 2016·Annual Review of Genetics·Kamal Kishore Joshi, Peter Chien
May 29, 2018·Applied and Environmental Microbiology·Issam HamdallahJoan L Slonczewski
Feb 23, 2017·Antimicrobial Agents and Chemotherapy·Erik SnesrudPatrick McGann
Apr 13, 2018·Annual Review of Biochemistry·Samar A Mahmoud, Peter Chien
May 10, 2019·International Journal of Molecular Sciences·Carlos Moreno-CinosKoen Augustyns
Apr 13, 2019·Genes & Development·Victoria DorichAlexandra M Deaconescu
May 7, 2020·Nucleic Acids Research·Katarzyna DubielJames L Keck
Oct 4, 2014·The Journal of Biological Chemistry·Yoshiharu SatoTomoko Yamamoto
Apr 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jinki YeomEduardo A Groisman
Jun 15, 2019·The Journal of Biological Chemistry·Susan Gottesman
Nov 5, 2016·The Journal of Biological Chemistry·Sophie BouilletChantal Iobbi-Nivol
Jul 12, 2019·Frontiers in Cellular and Infection Microbiology·Nina PennetzdorferStefan Schild
Jul 28, 2017·Frontiers in Molecular Biosciences·Alexander K W ElsholzKürşad Turgay
Feb 19, 2021·Protein Science : a Publication of the Protein Society·Jacob SchwartzAlexandra M Deaconescu
May 19, 2018·ACS Chemical Biology·Vaibhav BhandariWalid A Houry

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.