Anti-adipogenic activity of a new cyclic diarylheptanoid isolated from Alnus japonica on 3T3-L1 cells via modulation of PPARγ, C/EBPα and SREBP1c signaling

Bioorganic & Medicinal Chemistry Letters
Sang Hyun Sung, Mina Lee

Abstract

Total methanolic extract of Alnus japonica fruits exhibited significant anti-adipogenic activities in 3T3-L1 cells. A new cyclic diarylheptanoid (1) along with ten known compounds (2-11) were isolated by activity-guided fractionation. Compound 1, determined to be 4-hydroxy-alnus-3,5-dione, showed the most potent anti-adipogenic effect. Compound 1 significantly down-regulated expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and sterol regulatory element binding protein 1 (SREBP1c) in 3T3-L1 cells, as determined by quantitative real-time PCR and Western blot analysis. Furthermore, compound 1 suppressed mRNA expression of C/EBPβ and C/EBPδ during the early stage of adipogenesis as well as stearoyl coenzyme A desaturase 1 (SCD-1) and fatty acid synthase (FAS), target genes of SREBP1c. Upon investigating the mechanism of natural products, we propose that cyclic diarylheptanoid (1), the most potent constituent of A. japonica, can be a potent therapeutic agent against obesity through anti-adipogenesis via down-regulation of PPARγ, C/EBPα, and SREBP1c signaling.

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Mar 8, 2013·Bioorganic & Medicinal Chemistry Letters·Mina LeeSang Hyun Sung

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Citations

Oct 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoon Hee LeeHocheol Kim
Nov 20, 2016·BMC Complementary and Alternative Medicine·Hye-Youn KimSuntaek Hong
May 26, 2017·Natural Product Research·Xuguang ZhangJunqing Zhang
Aug 3, 2019·Archives of Physiology and Biochemistry·Ahmet MenteseAhmet Alver
Jul 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyejin LeeJae-Ha Ryu
Jul 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yajie CaiRunping Liu

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