PMID: 6164060Dec 1, 1980Paper

Anti-AMP antibody precipitation of multiply adenylylated forms of glutamine synthetase from Escherichia coli: a model relating both concentration and density of antigenic sites with the antibody-antigen interaction

Proceedings of the National Academy of Sciences of the United States of America
R J HohmanE R Stadtman

Abstract

Sheep antibodies directed against an AMP-bovine serum albumin conjugate exhibit highly specific binding toward AMP. These antibodies bind to the AMP moiety of adenylylated glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from Escherichia coli and to no other antigenic determinant on the protein. E coli glutamine synthetase can exist in variously modified (isomeric) forms that differ with respect to the number (0-12) and the distribution of identically adenylylated subunits [Ciardi, J. E., Cimino, F. & Stadtman, E. R. (1973) biochemistry 12, 4321-4330]. Using this enzyme, together with the AMP-specific antibodies, we have investigated the effects of the total concentration, population density, and topographical distribution of multiple identical antigenic determinants on the antigen-antibody interaction. Stopped flow fluorescence measurements show that the rate and extent of initial binding of the antibodies to the antigen are a function of the total concentration of AMP groups and are independent of the number of AMP groups der dodecamer. However, the rate of lattice formation increases with increasing epitope density, and the maximal amount of glutamine synthetase precipitated is directly proportiona...Continue Reading

References

Jun 14, 1978·Biochemical and Biophysical Research Communications·R J Hohman, E R Stadtman
Mar 1, 1972·Immunochemistry·D M Crothers, H Metzger
Jul 1, 1974·Archives of Biochemistry and Biophysics·R E MillerE R Stadtman
Mar 1, 1972·Immunochemistry·C L Hornick, F Karuch
Sep 26, 1966·Archives of Biochemistry and Biophysics·C A WoolfolkE R Stadtman
Oct 1, 1968·The Journal of Clinical Endocrinology and Metabolism·D RodbardG T Ross
Jul 1, 1964·Biochemistry·H N EISEN, G W SISKIND

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Citations

Jun 12, 1990·Journal of Immunological Methods·P L LeenaertsM Waer
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H K Chung, S G Rhee
Apr 30, 1985·Biochemical and Biophysical Research Communications·Y IkariyamaM Aizawa
Oct 5, 2001·The Journal of Biological Chemistry·E R Stadtman

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