PMID: 25773854Mar 17, 2015Paper

Anti-angiogenic activity of gecko aqueous extracts and its macromolecular components in CAM and HUVE-12 cells

Asian Pacific Journal of Cancer Prevention : APJCP
Zhen TangChun-Mei Wang

Abstract

Gecko is a kind of traditional Chinese medicine with remarkable antineoplastic activity. However, undefined mechanisms and ambiguity regarding active ingredients limit new drug development from gecko. This study was conducted to assess anti-angiogenic properties of the aqueous extracts of fresh gecko (AG) or macromolecular components separated from AG (M-AG). An enzyme-linked immunosorbent assay (ELISA) approach was applied to detect the vascular endothelial growth factor (VEGF) secretion of the tumor cells treated with AG or M-AG. The effect of AG or M-AG on vascular endothelial cell proliferation and migratory ability was analyzed by tetrazolium dye colorimetric method, transwell and wound-healing assays. Chick embryo chorioallantoic membrane (CAM) assays were used to ensure the anti-angiogenic activity of M-AG in vivo. The results showed that AG or M-AG inhibited the VEGF secretion of tumor cells, the relative inhibition rates of AG and M-AG being 27.2% and 53.2% respectively at a concentration of 20 μL/mL. AG and M-AG inhibited the vascular endothelial (VE) cell proliferation with IC50 values of 11.5 ± 0.5 μL/mL and 12.9 ± 0.4 μL/mL respectively. The VE cell migration potential was inhibited significantly (p<0.01) by the AG...Continue Reading

References

Jan 23, 1987·Science·J Folkman, M Klagsbrun
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
Mar 1, 1972·Annals of Surgery·J Folkman
Jan 1, 2003·Clinical Chemistry·Robert AuerbachNasim Akhtar
Jul 27, 2012·Molecular and Cellular Biochemistry·Yuan-Lei LouZhi-Feng Deng
Nov 3, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ae-Jin JeongJin Woong Chung
Oct 16, 2013·Chinese Journal of Integrative Medicine·Yan Sun
Dec 4, 2013·Biomedical Optics Express·Sung-Liang ChenXueding Wang
Dec 24, 2013·Cancer Treatment Reviews·Gabriel Limaverde-SousaCarlos Gil Ferreira

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