Anti-angiogenic effect of adiponectin in human primary microvascular and macrovascular endothelial cells

Microvascular Research
Karthikka PalanisamySubbulakshmi Chidambaram

Abstract

Neovascularization in retina and choroid involves interplay of many cytokines and growth factors. Vascular endothelial growth factor (VEGF) being a pro-angiogenic molecule has been found to be high in aqueous and vitreous humour of patients with proliferative diabetic retinopathy (PDR). VEGF is also found in the fibroblast and retinal pigment epithelial cells (RPE) of choroidal neovascular (CNV) membranes isolated from patients. Though anti-VEGF agents cause regression of clinically visible new vessels, there is evidence that they increase the occurrence of retinal tractional detachment and other adverse effects in PDR and CNV treatments. Adiponectin (APN) is a cytokine, found to be involved in the pathobiology of PDR. It is unclear whether APN plays a reparative or pathological role in the disease condition. In this study, we explored the effect of APN on tube formation in the primary culture of human umbilical vein macrovascular endothelial cells (HUVEC), human retinal microvascular endothelial cells (hREC) and human choroidal endothelial cells (hCEC). Anti-VEGF agent, bevacizumab (avastin) was used as a control. Full-length pAc-APN transfected in HUVEC, hRECs and hCECs inhibited basal tube formation and migration comparable ...Continue Reading

Citations

Apr 16, 2019·Journal of Cellular Physiology·Rong LiXiaodi Wang
Feb 13, 2020·Investigative Ophthalmology & Visual Science·Yuhang YangGuoming Zhang
Sep 20, 2019·Oncotarget·Flavia Alejandra BrunaVirginia Pistone-Creydt
Mar 29, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jennifer H Hammel, Evangelia Bellas
Aug 8, 2020·Frontiers in Pharmacology·Jingzan Wei, Yanyan Zhao
Feb 27, 2020·International Journal of Molecular Sciences·Zhongjie FuLois E H Smith
Mar 12, 2021·Pharmacology Research & Perspectives·María L FormicaSantiago D Palma
Apr 23, 2021·Life Sciences·Debrup ChakrabortyJing Wang
Nov 1, 2021·Endocrinology·Gregory P Westcott, Evan D Rosen

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