Anti-angiogenic effect of metformin in mouse oxygen-induced retinopathy is mediated by reducing levels of the vascular endothelial growth factor receptor Flk-1

PloS One
Soo Geun JoeJae-Young Koh

Abstract

To evaluate the effect of metformin on vascular changes in oxygen-induced retinopathy (OIR) in mouse, and to elucidate the possible underlying mechanism. OIR mice were treated with metformin by intraperitoneal injection from postnatal day 12 (P12) to P17 or P21. At P17 and P21, vessel formation and avascular areas were assessed using retinal flat mounts. Levels of vascular endothelial growth factor (VEGF) were measured by enzyme-linked immunosorbent assays, and the effects of metformin on VEGF-induced proliferation of human umbilical vein endothelial cells (HUVECs) were assessed. The effects of metformin on the levels of Flk1 (VEGF receptor-2) and phosphorylated Flk1 (pFlk1) were measured by Western blotting (HUVECs) and immunohistochemistry (retinal tissue). Retinal morphologic changes were analyzed between two groups (saline-treated OIR; metformin-treated OIR). Metformin treatment did not change the extent of avascular areas at P17. However, at P21, when OIR pathology was markedly improved in the saline-treated group, OIR pathology still remained in the metformin-treated OIR group. VEGF expression levels did not differ between metformin- and saline-treated OIR groups at P17 and P21, but Flk1 levels were significantly reduced ...Continue Reading

References

Jan 1, 1995·Annual Review of Cell and Developmental Biology·W Risau, I Flamme
Apr 26, 2005·BMJ : British Medical Journal·Josie M M EvansAndrew D Morris
Jan 29, 2008·Recent Patents on Anti-cancer Drug Discovery·Alfredo BudillonMichele Caraglia
Mar 25, 2008·Diabetes Research and Clinical Practice·Canan ErsoySazi Imamoglu
Apr 19, 2008·Breast Cancer Research and Treatment·Sirwan M HadadAlastair M Thompson
Apr 21, 2009·Gastroenterology·Donghui LiJames L Abbruzzese
Aug 8, 2009·American Journal of Ophthalmology·Jonathan A MicieliAndrew F Smith
Jan 8, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·D O XavierA V Belo
Feb 18, 2011·The New England Journal of Medicine·Helen A Mintz-HittnerUNKNOWN BEAT-ROP Cooperative Group
May 3, 2011·Neoplasia : an International Journal for Oncology Research·Ramandeep RattanViji Shridhar
Oct 5, 2011·Neonatology·José Carlos RiveraSylvain Chemtob
Nov 29, 2011·Clinical Science·Benoit ViolletFabrizio Andreelli
Feb 23, 2012·Current Diabetes Reviews·Gian P Giuliari
Mar 30, 2012·The New England Journal of Medicine·David A AntonettiThomas W Gardner
May 12, 2012·Cancer Discovery·Matthew J MartinRichard Marais
Jul 4, 2012·Oncology Reports·Buchu WuWen Di
Oct 4, 2012·Ophthalmology·Joan W MillerNapoleone Ferrara
Mar 15, 2013·Clinical Science·Alan W StittTimothy M Curtis
Oct 12, 2013·Cancer Treatment and Research·Alessandra LeoneAlfredo Budillon
Oct 17, 2013·Carcinogenesis·Tunde AkinyekeXin Li
Jan 15, 2014·Carcinogenesis·Katiuscia DallaglioAdriana Albini
Feb 25, 2014·ISRN Ophthalmology·Joanna M TarrRakesh Chibber
Feb 27, 2014·Biochimica Et Biophysica Acta·Daniele Lettieri BarbatoMaria Rosa Ciriolo
Mar 7, 2014·Biomolecules & Therapeutics·Masabumi Shibuya

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Citations

Dec 13, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kunal ManiarDibyajyoti Banerjee
Oct 15, 2018·Journal of Molecular Histology·Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany
May 6, 2019·Investigative Ophthalmology & Visual Science·Shinsuke NakamuraMasamitsu Shimazawa
Dec 23, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Gang WangMao Luo
Sep 29, 2019·European Journal of Pharmacology·Qiang MaJi-Chang Wang
Apr 3, 2021·SAGE Open Medicine·Yu Ren, Hua Luo
Apr 14, 2021·Ophthalmology and Therapy·Dewi Fathin RomdhoniyyahNicholas A V Beare
May 1, 2021·International Journal of Molecular Sciences·Koki WatanabeTsutomu Nakahara

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Methods Mentioned

BETA
ELISA
Assay
electrophoresis
Immunoprecipitation
ubiquitination

Software Mentioned

JAVA
SPSS
Sigma plot
Image J

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