Anti-angiogenic effects of thalidomide: expression of apoptosis-inducible active-caspase-3 in a three-dimensional collagen gel culture of aorta

Histochemistry and Cell Biology
Keiko FujitaHans-Joachim Merker

Abstract

The anti-angiogenic properties of thalidomide have led to the use of the agent as a remedy for multiple myeloma. Nevertheless, the anti-angiogenic moiety of thalidomide remains unidentified. In this study we examined the anti-angiogenic effects of thalidomide in an in vitro model using a three-dimensional collagen gel culture. Angiogenesis was significantly inhibited when the culture was treated with thalidomide plus cytochrome P-450 (CYP2B4), and the migrating cells and tubules were positive for active-caspase-3 in an accompanying immunohistochemical investigation. Transmission electron microscopic observation also confirmed that active-caspase-3-positive cells demonstrated apoptotic characteristics. This study is the first to morphologically demonstrate the effect of thalidomide in directly inducing the apoptosis of new tubules and migrating cells on a three-dimensional collagen gel culture of aorta. Taken together with earlier findings, our new results indicate that the thalidomide-induced inhibition of angiogenesis involves apoptosis in addition to the suppression of TNF-alpha and inhibition of cell migration from aorta explants, i.e., the factors important for capillarogenesis.

Citations

Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Nov 18, 2005·Histochemistry and Cell Biology·Keiko FujitaMasumi Akita
Nov 12, 2005·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Oct 6, 2006·Expert Review of Anticancer Therapy·Noopur RajeKenneth C Anderson
Feb 25, 2009·Expert Opinion on Pharmacotherapy·Christine Galustian, Angus Dalgleish
Jan 26, 2006·Expert Opinion on Investigational Drugs·Teru HideshimaKenneth C Anderson
Jun 25, 2005·Gynecologic Oncology·Levi S DownsSundaram Ramakrishnan
Feb 14, 2012·Expert Opinion on Pharmacotherapy·Sonia ValletKlaus Podar
Jan 1, 2014·Acta Biochimica Et Biophysica Sinica·Xiubao ChangA Keith Stewart

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved