Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs

Oncotarget
Dongmei YuanJiwu Wei

Abstract

Short interfering RNA (siRNA) targeting angiogenic factors and further inhibiting tumor angiogenesis, is one of the potent antitumor candidates for lung cancer treatment. However, this strategy must be combined with other therapeutics like chemotherapy. In this study, we designed a 5'-triphosphate siRNA targeting VEGF (ppp-VEGF), and showed that ppp-VEGF exerted three distinct antitumor effects: i) inhibition of tumor angiogenesis by silencing VEGF, ii) induction of innate immune responses by activating RIG-I signaling pathway, and thus activate antitumor immunity, iii) induction of apoptosis. In a subcutaneous model of murine lung cancer, ppp-VEGF displayed a potent antitumor effect. Our results provide a multifunctional antitumor molecule that may overcome the shortages of traditional antiangiogenic agents.

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Citations

Sep 16, 2018·Scientific Reports·Lina ZhangChengjin Zhang
Aug 30, 2016·Cancer Research·Lindsey EldridgeElizabeth M Wagner
Jan 20, 2018·Bioconjugate Chemistry·Christian R PalmerJohn T Wilson
Nov 20, 2021·The FEBS Journal·Madeleine R Smith, Guilherme Costa

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Methods Mentioned

BETA
in vitro transcription
ELISA
flow cytometry
electrophoresis

Software Mentioned

GraphPad Prism5

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