Anti-apoptotic action of Wnt5a in dermal fibroblasts is mediated by the PKA signaling pathways

Cellular Signalling
Kosuke ToriiMasaaki Matsuoka

Abstract

Wnts are secreted glycoproteins that control diverse biological processes, such as proliferation, differentiation, and apoptosis. We here found that Wnt5a inhibited apoptosis induced by serum deprivation in primary-cultured human dermal fibroblasts. Anti-apoptotic activity of Wnt5a was not inhibited by a dickkopf-1 (DKK), which blocks the canonical Wnt pathway. On the other hand, loss of function of protein kinase A (PKA), induced by treatment with PKA inhibitors, siRNA-mediated knocking down of endogenous PKA catalytic subunits, or enforced expression of dominant-negative PKA inhibited the Wnt5a anti-apoptotic activity, indicating the involvement of PKA in the Wnt5a anti-apoptotic activity. In agreement, phosphorylation levels of a cAMP response element binding protein (CREB), a representative downstream effector of PKA, the activation of which is known to lead to the pro-survival effects, was elevated by Wnt5a. In addition, Wnt5a increased the nuclear beta-catenin level and treatment with imatinib or ionomycin, either of which blocks the beta-catenin pathway, reduced the anti-apoptotic activity of Wnt5a, together suggesting the simultaneous involvement of the beta-catenin-mediated pathway in the Wnt5a anti-apoptotic activity....Continue Reading

References

Feb 7, 1997·Cell·M D JacobsonM C Raff
Jul 1, 1997·The EMBO Journal·H AberleR Kemler
Aug 15, 1997·The Biochemical Journal·G M Cohen
Sep 4, 1998·Science·T C HeK W Kinzler
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ShtutmanA Ben-Ze'ev
Sep 24, 1999·Biochemical and Biophysical Research Communications·M WrightJ Papkoff
Jun 20, 2000·Trends in Genetics : TIG·M KühlR T Moon
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·X FangG B Mills
Nov 30, 2000·Molecular and Cellular Biology·M LiK A Heidenreich
Sep 7, 2001·Current Opinion in Cell Biology·P N Adler, H Lee
Nov 14, 2001·The Journal of Biological Chemistry·Keitaro TsujiMasaaki Matsuoka
Jun 1, 2002·Science·Randall T MoonNorbert Perrimon
Apr 5, 2003·Mechanisms of Development·Beate KilianCarl Philipp Heisenberg
Sep 12, 2003·Developmental Cell·Michael T VeemanRandall T Moon
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Jun 3, 2005·Carcinogenesis·Chi Hoon ParkChul Hak Yang
Oct 6, 2005·Cancer Research·Hongying Wang, Wallace K MacNaughton
Nov 3, 2005·The Journal of Clinical Endocrinology and Metabolism·Annavarapu Srinivas RaoGeorg Brabant
Aug 22, 2006·The Journal of Biological Chemistry·Li Ma, Hsien-yu Wang
Oct 13, 2006·Molecular Biology of the Cell·T Néstor H MasckauchánJan Kitajewski

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Citations

Aug 22, 2013·The Journal of Physiological Sciences : JPS·Hiral AminSusan Tsivitse Arthur
Dec 17, 2009·The Journal of Biological Chemistry·Christian HundsruckerEnno Klussmann
Aug 5, 2009·The Journal of Biological Chemistry·Christian HansenTommy Andersson
May 6, 2010·The Journal of Biological Chemistry·Victor S C WongPatricia L Brubaker
Mar 16, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jeong Eun KimSung Eun Chang
Mar 3, 2009·American Journal of Respiratory Cell and Molecular Biology·Louis J VugaNaftali Kaminski
Oct 31, 2015·Cellular and Molecular Life Sciences : CMLS·Kuldeep Kumawat, Reinoud Gosens
Oct 19, 2011·Journal of Dermatological Science·Alvin Wen Choong ChuaToan Thang Phan
Apr 2, 2010·The Journal of Investigative Dermatology·Chi-Hyun ParkJin Ho Chung
Dec 17, 2010·The Journal of Investigative Dermatology·Alvin W C ChuaToan T Phan
Aug 27, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Merry Jo OurslerSundeep Khosla
Oct 17, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Marike M van BeugeRuud A Bank
May 23, 2014·Molecular and Cellular Endocrinology·R Formosa, J Vassallo
Sep 10, 2011·Acta Biochimica Et Biophysica Sinica·Antara De
Jul 16, 2009·British Journal of Cancer·S L McDonald, A Silver
Jun 27, 2020·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hye Jin HeoChi Dae Kim
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Malini Sen, Gourisankar Ghosh
Mar 15, 2014·International Journal of Oncology·Lee Fah YapAlan Soo Beng Khoo
Sep 17, 2009·Journal of Cell Science·Cordula Surmann-SchmittMichael Stock
Apr 28, 2021·JCI Insight·Li-Mei LiangWan-Li Ma

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