Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes

Stem Cell Reports
Ah Reum LeeDong Ryul Lee

Abstract

Cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cell nuclear transferred (SCNT) mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the recovery effects of melatonin on cryo-damage in cloned embryos. Development of SCNT embryos using cryopreserved oocyte cytoplasm (SCNT-CROC) was inferior to those using fresh cytoplasm (SCNT-FOC). Using RNA-sequencing analysis, we found upregulation of eight pro-apoptotic-related genes (Cyct, Dapk2, Dffb, Gadd45g, Hint2, Mien1, P2rx7, and Pmaip) in the SCNT-CROC group. Furthermore, the addition of melatonin, an agent that reduces apoptosis and ROS production, enhanced blastocyst formation rates in the SCNT-CROP group when compared with the melatonin-untreated group. Additionally, melatonin treatment increased the derivation efficiency of pluripotent stem cells from cloned embryos using cryopreserved oocyte.

Citations

Aug 3, 2019·Bioscience Reports·Jia Soon LenShi-Xiong Tan
May 26, 2021·Molecular Reproduction and Development·Jiehuan XuJianjun Dai
Jul 25, 2021·International Journal of Molecular Sciences·Alexander G Kuzma-HuntLaura A Favetta

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Methods Mentioned

BETA
RNA-seq
in vitro transcription
confocal microscopy
light microscopy

Software Mentioned

R
ImageJ
Cufflinks
SAS

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