Anti-apoptotic role of EGF in HaCaT keratinocytes via a PPARbeta-dependent mechanism

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Pengfei LiangXiaoyuan Huang

Abstract

Epidermal growth factor (EGF) plays an important role in epithelial cell proliferation and apoptosis. Our recent studies found that EGF-attenuated tumor necrosis factor-alpha induced HaCaT keratinocyte apoptosis, and this effect was accompanied by up-regulation of the expression of peroxisome proliferator-activated receptor beta (PPARbeta). However, little is known about whether PPARbeta is functionally involved in the inhibition of keratinocyte apoptosis by EGF. Here, we showed that EGF up-regulated the DNA-binding and transcriptional regulation activities of PPARbeta. Antisense phosphorothioate oligonucleotides against PPARbeta markedly inhibited de novo synthesis of PPARbeta and attenuated the protective effect of EGF on tumor necrosis factor-alpha-induced apoptosis. L165041, a specific PPARbeta ligand, significantly enhanced the transcriptional regulation activity of PPARbeta and increased the protective effect of EGF. These results suggest a molecular mechanism by which EGF protects HaCaT keratinocytes against apoptosis in a PPARbeta-dependent manner.

References

Jun 6, 2000·Nature·S KerstenW Wahli
Apr 1, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiro UmedaJunji Takeda
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Nicolas Di-PoïWalter Wahli
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·F Gregory BuchananRaymond N DuBois
Jan 16, 2007·Biochemical and Biophysical Research Communications·Zhen Cheng YanZhi Ming Zhu
Jul 26, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Emiko Aiba-KojimaKotaro Yoshimura
Jul 27, 2007·Journal of Cellular Physiology·Olav F DajaniThoralf Christoffersen
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jan Jeroen VranckxFeng Yao
Nov 22, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Tracy Jane Stefonek, Kristyn S Masters
Jan 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Dipak PanigrahyMark W Kieran
Feb 16, 2008·Circulation Research·Sheng Zhong DuanRichard M Mortensen

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Citations

Aug 16, 2016·Biochemical and Biophysical Research Communications·Swati AgarwalRajnish Kumar Chaturvedi
Jul 20, 2011·Biomacromolecules·Christopher A VaianaMadhavi P Kadakia
Dec 19, 2019·Dermatologic Therapy·Oktay KayaBilgehan Aydın

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis