Anti-apoptotic therapy with a Tat fusion protein protects against excitotoxic insults in vitro and in vivo.

Experimental Neurology
Kevin L JuRobert M Sapolsky

Abstract

A number of gene therapy approaches have been developed for protecting neurons from necrotic neurological insults. Such therapies are limited by the need for transcription and translation of the protective protein, delaying therapeutic impact. As an alternative, we explore the neuroprotective potential of protein therapy, using a fusion protein comprised of the death-suppressing BH4 domain of the Bcl-xL protein and the protein transduction domain of the human immunodeficiency virus Tat protein. This fusion protein decreased neurotoxicity caused by the excitotoxins glutamate and kainic acid in primary hippocampal cultures, and decreased hippocampal damage in vivo in an excitotoxic seizure model.

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Citations

Dec 6, 2008·Biochimica Et Biophysica Acta·Yi-Ping RongClark W Distelhorst
Mar 3, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Tobias EngelDavid C Henshall
Jun 23, 2010·Journal of Experimental & Clinical Cancer Research : CR·Wei ZhangSongbin Fu

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