Anti-arthritic effects of magnolol in human interleukin 1β-stimulated fibroblast-like synoviocytes and in a rat arthritis model.

PloS One
Jyh-Horng WangChia-Ron Yang

Abstract

Fibroblast-like synoviocytes (FLS) play an important role in the pathologic processes of destructive arthritis by producing a number of catabolic cytokines and metalloproteinases (MMPs). The expression of these mediators is controlled at the transcriptional level. The purposes of this study were to evaluate the anti-arthritic effects of magnolol (5,5'-Diallyl-biphenyl-2,2'-diol), the major bioactive component of the bark of Magnolia officinalis, by examining its inhibitory effects on inflammatory mediator secretion and the NF-κB and AP-1 activation pathways and to investigate its therapeutic effects on the development of arthritis in a rat model. The in vitro anti-arthritic activity of magnolol was tested on interleukin (IL)-1β-stimulated FLS by measuring levels of IL-6, cyclooxygenase-2, prostaglandin E(2), and matrix metalloproteinases (MMPs) by ELISA and RT-PCR. Further studies on how magnolol inhibits IL-1β-stimulated cytokine expression were performed using Western blots, reporter gene assay, electrophoretic mobility shift assay, and confocal microscope analysis. The in vivo anti-arthritic effects of magnolol were evaluated in a Mycobacterium butyricum-induced arthritis model in rats. Magnolol markedly inhibited IL-1β (10 ...Continue Reading

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Citations

Apr 10, 2015·Journal of Cellular and Molecular Medicine·Wei-Ping ChenLi-Dong Wu
Jan 7, 2015·Environmental Toxicology and Pharmacology·A-Rang ImSungwook Chae
Jul 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ling ZhaoZhao-Xiang Bian
Jul 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Youn-Hwan HwangHyunil Ha
Jun 3, 2016·Chemistry & Biodiversity·Luis Cezar RodriguesRoberto Mioso
Apr 6, 2021·Frontiers in Pharmacology·Yiping LinYing Song

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Methods Mentioned

BETA
ELISA
confocal
nuclear translocation
electrophoretic mobility shift assay
confocal microscopy
transfection
PCR

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