Anti-bacterial antibody and T cell responses in bronchiectasis are differentially associated with lung colonization and disease

Respiratory Research
Fathia G JaatStephen M Todryk

Abstract

As a way to determine markers of infection or disease informing disease management, and to reveal disease-associated immune mechanisms, this study sought to measure antibody and T cell responses against key lung pathogens and to relate these to patients' microbial colonization status, exacerbation history and lung function, in Bronchiectasis (BR) and Chronic Obstructive Pulmonary Disease (COPD). One hundred nineteen patients with stable BR, 58 with COPD and 28 healthy volunteers were recruited and spirometry was performed. Bacterial lysates were used to measure specific antibody responses by ELISA and T cells by ELIspot. Cytokine secretion by lysate-stimulated T cells was measured by multiplex cytokine assay whilst activation phenotype was measured by flow cytometry. Typical colonization profiles were observed in BR and COPD, dominated by P.aeruginosa, H.influenzae, S.pneumoniae and M.catarrhalis. Colonization frequency was greater in BR, showing association with increased antibody responses against P.aeruginosa compared to COPD and HV, and with sensitivity of 73% and specificity of 95%. Interferon-gamma T cell responses against P.aeruginosa and S.pneumoniae were reduced in BR and COPD, whilst reactive T cells in BR had similar...Continue Reading

References

Nov 24, 2001·American Journal of Respiratory and Critical Care Medicine·J AngrillA Torres
Jun 7, 2005·American Journal of Respiratory and Critical Care Medicine·Andrew K SullivanAndrew P Fontenot
May 1, 2007·Respiratory Medicine·Paul T KingPeter W Holmes
Jun 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gerd DöringUNKNOWN Flagella Vaccine Trial Study Group
Sep 23, 2008·Clinical and Experimental Immunology·P T KingS R Holdsworth
Aug 7, 2009·European Journal of Immunology·Stephen M TodrykAdrian V S Hill
Jul 16, 2010·Thorax·M C PasteurUNKNOWN British Thoracic Society Non-CF Bronchiectasis Guideline Group
Mar 3, 2011·PloS One·John R Erb-DownwardGary B Huffnagle
Jun 23, 2012·American Journal of Respiratory and Critical Care Medicine·Weihui WuGregory P Priebe
Jun 30, 2012·American Journal of Respiratory and Critical Care Medicine·James D ChalmersAdam T Hill
Nov 13, 2012·The Journal of Allergy and Clinical Immunology·Paul T KingStephen R Holdsworth
Jan 26, 2013·American Journal of Respiratory and Critical Care Medicine·Michael M TunneyJ Stuart Elborn
Mar 19, 2013·American Journal of Respiratory and Critical Care Medicine·Keven M Robinson, John F Alcorn
Dec 18, 2013·American Journal of Respiratory and Critical Care Medicine·James D ChalmersAdam T Hill
Mar 7, 2014·Annals of the American Thoracic Society·Geraint B RogersDavid J Serisier
May 16, 2014·American Journal of Respiratory and Critical Care Medicine·Suresh Gopi KalathilYasmin Thanavala
Jun 20, 2014·Immunological Reviews·Jeremy P McAleer, Jay K Kolls
Aug 16, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Claire AguilarOlivier Lortholary
Aug 26, 2014·Lancet·Robert P DicksonGary B Huffnagle
Nov 26, 2014·Nature Reviews. Immunology·Benjamin J Marsland, Eva S Gollwitzer
Mar 20, 2015·Clinical and Vaccine Immunology : CVI·Timothy F Murphy
Mar 20, 2015·American Journal of Respiratory and Critical Care Medicine·Kathryn J QuigleyRosemary J Boyton
May 7, 2015·American Journal of Respiratory and Critical Care Medicine·Marc A SzeJames C Hogg
Mar 17, 2016·Annual Review of Pathology·Rosemary J Boyton, Daniel M Altmann
Oct 22, 2016·The Lancet Global Health· The Lancet Global Health
Nov 20, 2016·The Lancet. Respiratory Medicine·Melissa J McDonnellJames D Chalmers
Jun 15, 2017·Respiratory Medicine·Guillermo Suarez-CuartinJames D Chalmers

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Citations

Sep 24, 2020·The Clinical Respiratory Journal·Liyang LiChangzhou Shao
Jul 15, 2021·Seminars in Respiratory and Critical Care Medicine·Holly R Keir, James D Chalmers
Dec 2, 2021·International Immunology·Visai Muruganandah, Andreas Kupz

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Methods Mentioned

BETA
ELISA

Software Mentioned

ELIspot

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