Anti-benzopyrene-7,8-diol-9,10-epoxide induces apoptosis via mitochondrial pathway in human bronchiolar epithelium cells independent of the mitochondria permeability transition pore

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Hao SangJue Li

Abstract

Anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) is a ubiquitous environmental pollutant contained in tobacco smoke, automobile exhausts and barbecued foods. The carcinogenicity of BPDE on animals has been well characterized, whereas its apoptotic effect is not well defined. A recent study has shown that BPDE-mediated apoptotic pathway has varying specificity across different cell lines. Squamous cell carcinoma (SCC) arises from bronchiolar epithelium cells, therefore, we set out to investigate the pulmonary toxicity and apoptotic effect of BPDE in human bronchiolar epithelium cells (16HBE). Our results show BPDE induces mitochondrial-mediated apoptosis in a dose-dependent manner in 16HBE cells. The cleavage of caspase-3,-9 and release of Cytochrome c (cyt c) was regulated during apoptotic stimulation. However, the opening of mitochondria permeability transition pore (mPTP) has not been detected. Furthermore, our data also indicate that the formation of reactive oxygen species (ROS), decline of mitochondrial membrane potential (ΔΨ(m)), increasing p53 and decreasing c-Myc levels play important roles in response to BPDE toxicity. In conclusion, these results suggest that BPDE-mediated apoptosis occurs via caspase-9 dependent mito...Continue Reading

References

Mar 28, 2003·The Journal of Biological Chemistry·Susan Chalmers, David G Nicholls
Apr 13, 2004·Oncogene·Xavier SaelensPeter Vandenabeele
Feb 24, 2005·Cancer Biology & Therapy·Zhaoyu Jin, Wafik S El-Deiry
Oct 11, 2008·Cell Death and Differentiation·G KroemerUNKNOWN Nomenclature Committee on Cell Death 2009
Feb 2, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J FerlayE Steliarova-Foucher
Feb 9, 2010·Cell Adhesion & Migration·Marileila Varella-Garcia
Feb 11, 2010·Molecular Cancer Research : MCR·Hayla K SlussStephen N Jones
Nov 10, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tomoya KawaguchiHidefumi Sasaki
Apr 5, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marjo HuovinenKirsi H Vähäkangas
Jun 7, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Sung-Keum SeoIn-Chul Park
Jul 1, 2011·BMC Genomics·Hamza HamoucheneDavid H Phillips
Aug 2, 2011·American Journal of Respiratory and Critical Care Medicine·Balazs Halmos, Charles A Powell
Oct 6, 2011·Biochemical and Biophysical Research Communications·Alessandro Rufini, Gerry Melino
Nov 22, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Béatrice DendeléDominique Lagadic-Gossmann
Mar 6, 2012·Lancet·Yang Xie, John D Minna

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Citations

Sep 7, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F V DuarteC M Palmeira
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Gui-Chun DingZhong-Hua Shi
Apr 1, 2018·European Journal of Clinical Investigation·Christiana DemetriouPaolo Vineis
Oct 20, 2018·Environmental Science and Pollution Research International·Qin WeiLing Zhao

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