Anti-beta-crystallin antibodies (mouse) or sera from humans with age-related cataract are cytotoxic for lens epithelial cells in culture

Experimental Eye Research
N IbarakiT Shinohara

Abstract

Circulating autoantibodies against lens antigens are prevalent in patients with age-related cataract (ARC), but their pathogenic significance is unknown. We hypothesized that these autoantibodies are cytotoxic for lens epithelial cells (LECs). To test this hypothesis. We incubated LECs with mouse polyclonal or monoclonal antibodies against beta-crystallin (anti-beta) in the presence or absence of guinea pig complement. We found that anti-beta in the presence of the complement bound to and killed mouse LECs (MLECs) and human LECs (HLECs). Sera obtained from patients with ARC also were cytotoxic to both HLECs and MLECs in culture. Heat-inactivated human sera were not cytotoxic to LECs in the absence of the complement, but were cytotoxic to both HLECs and MLECs in the presence of additional complement. These results support the hypothesis that autoantibodies against lens antigens are cytotoxic to LECs, and that cell death may involve complement-mediated pathways.

Citations

May 10, 2000·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·T ShinoharaL T Chylack
Jun 10, 2008·Investigative Ophthalmology & Visual Science·Ling ChenLynn K Gordon
Nov 1, 2013·Veterinary Ophthalmology·Nobuyuki KanemakiMitsuyoshi Azuma
Nov 7, 2000·International Ophthalmology Clinics·J R Trevithick, K P Mitton
Sep 22, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Deshka DoychevaZierhut Manfred
Nov 30, 2002·Journal of Cataract and Refractive Surgery·Junko NakajimaYoshimitsu Tokunaga
Jan 7, 2000·Biochemical and Biophysical Research Communications·D P SinghT Shinohara

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