May 5, 2018

Anti-biofilm and Anti-Virulence Efficacy of Celastrol Against Stenotrophomonas maltophilia

International Journal of Medical Sciences
Hye-Rim KimYong-Bin Eom

Abstract

Stenotrophomonas maltophilia is a multi-drug resistant opportunistic pathogen that causes nosocomial infections in immunocompromised patients. This pathogen is difficult to treat owing to its intrinsic multidrug resistance and ability to form antimicrobial-tolerant biofilms. In the present study, we aimed to assess the potential use of celastrol as a novel anti-biofilm and/or anti-virulence agent against S. maltophilia. Results showed that celastrol at its sub-inhibitory doses decreased biofilm formation and disrupt the established biofilms produced by S. maltophilia. Celastrol-induced decrease in biofilm formation was dose-dependent based on the results of the microtiter plate biofilm assays and confocal laser scanning microscopy. In addition, our data validated the anti-virulence efficacy of celastrol, wherein it significantly interfered with the production of protease and motility of S. maltophilia. To support these phenotypic results, transcriptional analysis revealed that celastrol down-regulated the expression of biofilm- and virulence- associated genes (smeYZ, fsnR, and bfmAK) in S. maltophilia. Interestingly, celastrol significantly inhibited the expression of smeYZ gene, which encodes the resistance-nodulation-division...Continue Reading

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Mentioned in this Paper

Study
Gene Expression Regulation, Bacterial
Bacterial Proteins
Pathogenicity
Genes
Bacteriocidal Agents
Cell Motility
Pathogenic Organism
Transcription, Genetic
Peptide Hydrolases

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