Anti-breast cancer and anti-angiogenic potential of a lichen-derived small-molecule: barbatolic acid

Cytotechnology
Mehmet Varol

Abstract

Natural products have been used for centuries as the most potent remedies to cure many diseases including cancer diseases. Angiogenesis is defined as the formation of new capillaries from existing vessels and plays a key role in the tumorigenesis process. Barbatolic acid is a little known lichen-derived small-molecule. In the present study, barbatolic acid was isolated from the acetone extract of Bryoria capillaris, and its anti-breast cancer and anti-angiogenic potential was investigated using human umbilical vein endothelial cells (HUVECs), human breast ductal carcinoma (T-47D) and cisplatin-resistant BRCA2-mutated human breast TNM stage IV adenocarcinoma (HCC1428) cells. AlamarBlue™ cell viability, lactate dehydrogenase cellular membrane degradation and PicoGreen™ dsDNA quantitation assays were performed to determine the cytotoxic potential of barbatolic acid. Anti-angiogenic and anti-migratory activities were investigated using endothelial tube formation assay and scratch wound healing assay, respectively. Half maximal inhibitory concentration of barbatolic acid was found to be higher than 100 µM for HUVEC, HCC1428 and T-47D cells. The sub-cytotoxic concentrations such as 25 µM, 50 µM and 100 µM were applied to determine an...Continue Reading

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Citations

Jan 18, 2020·Biomolecules·Zuzana SolárováPeter Solár
Feb 16, 2021·Phytotherapy Research : PTR·Gönül Ulus
May 31, 2021·Seminars in Cancer Biology·Tanvir Ul Hassan DarShafiul Haque

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