Anti-C1q Autoantibodies from Systemic Lupus Erythematosus Patients Induce a Proinflammatory Phenotype in Macrophages

The Journal of Immunology : Official Journal of the American Association of Immunologists
Sophia Thanei, Marten Trendelenburg

Abstract

Anti-C1q autoantibodies (anti-C1q) are frequently found in patients with systemic lupus erythematosus (SLE) and correlate with the occurrence of proliferative lupus nephritis. A previous study of anti-C1q in experimental lupus nephritis demonstrated an important role for FcγRs in the pathogenesis of lupus nephritis, suggesting a direct effect on phagocytes. Therefore, we developed an in vitro model to study the effect of SLE patient-derived anti-C1q bound to immobilized C1q (imC1q) on human monocyte-derived macrophages (HMDMs) obtained from healthy donors and SLE patients. HMDMs were investigated by analyzing the cell morphology, LPS-induced cytokine profile, surface marker expression, and phagocytosis rate of apoptotic Jurkat cells. Morphologically, bound anti-C1q induced cell aggregations of HMDMs compared with imC1q or IgG alone. In addition, anti-C1q reversed the effect of imC1q alone, shifting the LPS-induced cytokine release toward a proinflammatory response. FcγR-blocking experiments revealed that the secretion of proinflammatory cytokines was mediated via FcγRII. The anti-C1q-induced inflammatory cytokine profile was accompanied by a downregulation of CD163 and an upregulation of LPS-induced CD80, CD274, and MHC class I...Continue Reading

References

Aug 1, 1991·Clinical Immunology and Immunopathology·H G HerrodM E Dockter
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Oct 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·I E CoremansC G Kallenberg
Mar 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M KolosovR W Leu
Mar 21, 2000·Rheumatology·M C Pickering, M J Walport
Aug 10, 2000·The Journal of Experimental Medicine·P R TaylorM J Walport
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J S NavratilJ M Ahearn
Apr 5, 2001·The New England Journal of Medicine·M J Walport
Aug 18, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·D J Nikolic-Paterson, R C Atkins
Sep 11, 2002·Cytokine & Growth Factor Reviews·Amy M BeebeRene de Waal Malefyt
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Jun 7, 2003·Journal of Autoimmunity·Luis Llorente, Yvonne Richaud-Patin
Jul 2, 2003·Journal of Clinical Pathology·C C Mok, C S Lau
Jan 17, 2004·Blood·Giuseppe CastellanoCees van Kooten
Feb 19, 2004·European Journal of Immunology·Masahide YamadaYuji Matsuzawa
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alma J NautaAnja Roos
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Apr 18, 2006·Journal of Leukocyte Biology·Deborah A FraserAndrea J Tenner
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy BergtoldRaphael Clynes
Mar 21, 2007·Journal of Autoimmunity·Udo S GaiplMartin Herrmann
Mar 27, 2007·Molecular Immunology·Eszter CsomorAnna Erdei
Oct 27, 2007·Molecular Immunology·L A TrouwP Gasque
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lena SchifferAnne Davidson
Jun 3, 2008·Journal of Autoimmunity·Ana LleoM Eric Gershwin
Oct 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deborah A FraserAndrea J Tenner
May 15, 2010·Journal of Biomedicine & Biotechnology·Desmond Yat Hin Yap, Kar Neng Lai
Sep 16, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Cornelia BiglerMarten Trendelenburg
Mar 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramalingam BethunaickanAnne Davidson
Nov 15, 2011·Journal of Immunological Methods·C A AmbarusD L P Baeten

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Citations

Mar 20, 2018·Genes and Immunity·Kozue Uchio-YamadaNoboru Manabe
Dec 14, 2019·Clinical Reviews in Allergy & Immunology·Federica DefendiChantal Dumestre-Pérard
Jun 11, 2019·Frontiers in Immunology·Wen-Tao MaDe-Kun Chen
Mar 28, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Qian WangMingshu Sun
May 2, 2018·Frontiers in Immunology·Jenny GiangJeffrey Damman
May 11, 2021·Clinical & Translational Immunology·Marten Trendelenburg

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