Anti-carbamylated protein antibodies precede disease onset in monkeys with collagen-induced arthritis

Arthritis Research & Therapy
Marije K VerheulLeendert A Trouw

Abstract

Rheumatoid factor (RF), anti-citrullinated protein antibodies (ACPA) and anti-carbamylated protein (anti-CarP) antibodies are rheumatoid arthritis (RA)-associated autoantibodies. Besides their presence in human serum, anti-CarP antibodies have also been described in rodent models of arthritis, while ACPA are not consistently detectable. Data on these RA-associated autoantibodies in primates are not available. Therefore, we investigated the presence of RF, anti-CarP antibodies and ACPA in rhesus monkeys before and after collagen-induced arthritis immunizations. In previous studies, arthritis was induced in groups of rhesus monkeys by immunisation with collagen following pre-treatment with placebo, abatacept or Roactemra. Previously collected serum was used to measure, autoantibodies by ELISA, detecting anti-CarP antibodies, RF-IgM and antibodies against CCP2, citrullinated myelin basic protein and citrullinated fibrinogen. Out of the three autoantibodies, only anti-CarP antibodies were detectable in resus monkeys with arthritis. RF-IgM and ACPA were undetectable and below the detection limit of the ELISA. The level of anti-CarP antibodies increases over time and, similar to in humans and mice, these autoantibodies were already d...Continue Reading

References

Jul 10, 2010·Arthritis and Rheumatism·Sanna TurunenJuha Risteli
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jing ShiLeendert A Trouw
Dec 6, 2016·Nature Reviews. Immunology·Vivianne MalmströmLars Klareskog
Mar 10, 2017·Nature Reviews. Rheumatology·Leendert A TrouwRene E M Toes
Apr 27, 2017·Annals of the Rheumatic Diseases·Jacqueline S DekkersRené E M Toes

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Methods Mentioned

BETA
ELISA
FCS
enzyme-linked
enzyme-linked immunosorbent assay

Software Mentioned

SPSS
Graphpad Prism

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