Anti-correlation of HER2 and focal adhesion complexes in the plasma membrane

PloS One
Florian WeinbergNiels de Jonge

Abstract

Excess presence of the human epidermal growth factor receptor 2 (HER2) as well as of the focal adhesion protein complexes are associated with increased proliferation, migratory, and invasive behavior of cancer cells. A cross-regulation between HER2 and integrin signaling pathways has been found, but the exact mechanism remains elusive. Here, we investigated whether HER2 colocalizes with focal adhesion complexes on breast cancer cells overexpressing HER2. For this purpose, vinculin or talin green fluorescent protein (GFP) fusion proteins, both key constituents of focal adhesions, were expressed in breast cancer cells. HER2 was either extracellularly or intracellularly labeled with fluorescent quantum dots nanoparticles (QDs). The cell-substrate interface was analyzed at the location of the focal adhesions by means of total internal reflection fluorescent microscopy or correlative fluorescence- and scanning transmission electron microscopy. Expression of HER2 at the cell-substrate interface was only observed upon intracellular labeling, and was heterogeneous with both HER2-enriched and -low regions. In contrast to an expected enrichment of HER2 at focal adhesions, an anti-correlated expression pattern was observed for talin and H...Continue Reading

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Citations

Dec 3, 2020·International Journal of Molecular Sciences·Florian WeinbergNiels de Jonge

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Methods Mentioned

BETA
electron microscopy
transmission electron microscopy
electron
transfection
light microscopy
fluorescence microscopy
Fluorescence

Software Mentioned

ImageJ
NIS Elements
GraphpadPrism7
ZenBlue
Gatan software Digital Micrograph
Fiji ImageJ
LAS FX
ZEISS

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