PMID: 7546609Aug 1, 1995Paper

Anti-DNA.RNA antibodies: an efficient tool for non-isotopic detection of Listeria species through a liquid-phase hybridization assay

Applied Microbiology and Biotechnology
I FlissS Pandian

Abstract

This study was undertaken to evaluate the potential of a new approach using anti-DNA.RNA monoclonal antibodies to detect Listeria in both pure culture and inoculated meat and meat products. A sensitive liquid-phase assay was first developed, based on the formation in solution of a hybrid between a 784-bp DNA probe, specific for the genus Listeria, and target rRNA. Monoclonal antibody and antisera raised against hybrid nucleic acids were then used in various immunoenzymatic assays to detect specific hybrids formed in solution. System 2, using a double sandwich enzyme-linked immunosorbent assay, and system 1, using a biotinylated probe, proved to be very effective. The method using biotin-streptavidin complex, however, resulted in a higher background signal. System 2 described here, using unlabeled probe, was more effective. This strategy allowed the detection of as little as 2.5 pg target RNA from pure culture and 500 cells from inoculated meat homogenate, even in the presence of other contaminating bacteria. The assay was more sensitive and could be completed within 3 h, as opposed to several days when conventional culture methods were used.

References

Dec 1, 1990·Applied and Environmental Microbiology·A R DattaJ Russell
Apr 1, 1989·Applied and Environmental Microbiology·S NotermansP Guinee
Jul 1, 1988·The Journal of Infection·R J LamontA P MacGowan
May 1, 1979·Journal of Food Protection·J L ButlerG C Smith
Oct 1, 1991·Journal of Food Protection·B NorrungN Skovgaard

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Citations

Jul 12, 2014·Expert Review of Molecular Diagnostics·Zonglin HuChristopher A Elkins

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