Anti-fibrogenic effects of the anti-microbial peptide cathelicidin in murine colitis-associated fibrosis

Cellular and Molecular Gastroenterology and Hepatology
Jun Hwan YooHon Wai Koon

Abstract

Cathelicidin (LL-37 in human and mCRAMP in mice) represents a family of endogenous antimicrobial peptides with anti-inflammatory effects. LL-37 also suppresses collagen synthesis, an important fibrotic response, in dermal fibroblasts. Here we determined whether exogenous cathelicidin administration modulates intestinal fibrosis in two animal models of intestinal inflammation and in human colonic fibroblasts. C57BL/6J mice (n=6 per group) were administered intracolonically with a trinitrobenzene sulphonic acid (TNBS) enema to induce chronic (6-7 weeks) colitis with fibrosis. mCRAMP peptide (5 mg/kg every 3 day, week 5-7) or cathelicidin gene (Camp)-expressing lentivirus (10(7) infectious units week 4) were administered intracolonically or intravenously, respectively. 129Sv/J mice were infected with Salmonella typhimurium orally to induce cecal inflammation with fibrosis. Camp expressing lentivirus (10(7) infectious units day 11) was administered intravenously. TNBS-induced chronic colitis was associated with increased colonic collagen (col1a2) mRNA expression. Intracolonic cathelicidin (mCRAMP peptide) administration or intravenous delivery of lentivirus-overexpressing cathelicidin gene significantly reduced colonic col1a2 mRNA ...Continue Reading

Citations

Apr 14, 2017·Current Opinion in Gastroenterology·Giovanni Latella, Florian Rieder
May 3, 2016·Inflammatory Bowel Diseases·Lihua SunHua Yang
Dec 3, 2014·Nature Reviews. Gastroenterology & Hepatology·Isobel Leake
Apr 28, 2016·Pharmacological Reports : PR·Adam FabisiakJakub Fichna
Jun 16, 2015·Mediators of Inflammation·Woo Seok YangJae Youl Cho
Jun 9, 2020·BioMed Research International·Binbin YangMing Q Wei
Feb 2, 2021·Journal of Inflammation Research·Paulina Krawiec, Elżbieta Pac-Kożuchowska
May 18, 2021·PloS One·Marta Kinga LemieszekJanusz Milanowski
Mar 9, 2020·Ecotoxicology and Environmental Safety·Marta Kinga LemieszekJanusz Milanowski
Mar 23, 2020·Dermatology : International Journal for Clinical and Investigative Dermatology·Hamideh MoravvejMehdi Gheisari

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Methods Mentioned

BETA
surgical resection
flow cytometry
Enzyme-Linked
enzyme-linked immunosorbent assay

Software Mentioned

Prism
GraphPad

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