Anti-fibrotic Role of miR-214 in Thioacetamide-induced Liver Cirrhosis in Rats

Toxicologic Pathology
Takeshi IzawaJyoji Yamate

Abstract

An increasing number of studies have focused on the role of microRNAs in liver fibrosis/cirrhosis. miR-214 has recently attracted more attention as a fibrosis-related factor; however, the molecular mechanisms in hepatic fibrogenesis remain largely unknown. Here, we investigate the pathological role of miR-214 during progression of liver cirrhosis in rats. Rats were injected intraperitoneally with thioacetamide at a dose of 100 mg/kg body weight, twice a week. The liver was collected at post first injection weeks 5, 10, 15, and 20. Hepatic expression of miR-214 was analyzed by real-time polymerase chain reaction, in situ hybridization, and laser microdissection. The effects of miR-214 overexpression were investigated by in vitro transfection using fibroblastic MT-9 cells. miR-214 was highly upregulated in the fibrotic area in parallel with the cirrhosis progression. miR-214 overexpression in MT-9 cells under transforming growth factor-β1 stimulation resulted in decreased cell number and increased expression of cleaved caspase 3 and decreased expression of α-smooth muscle actin, suggesting that miR-214 induces apoptosis and inhibits myofibroblast differentiation in fibroblastic cells under stimulation of fibrogenic factors. These...Continue Reading

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Apr 5, 2017·Bioscience, Biotechnology, and Biochemistry·Jae-Sang HongYoung Sik Lee
May 11, 2016·Pulmonary Circulation·Hannah C StevensLaura Denby
May 8, 2020·Molecular Medicine Reports·Shaohong WuJianhe Gan
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Sep 22, 2021·Pediatric Surgery International·Tomohisa YoneyamaHiroomi Okuyama

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Methods Mentioned

BETA
transfection
protein assay

Software Mentioned

WinRoof
Prism

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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