Anti-glycan IgM repertoires in newborn human cord blood

PloS One
Li Xia, Jeffrey C Gildersleeve

Abstract

Natural antibodies are an innate-like subset of serum antibodies involved in host defense, tumor surveillance, homeostasis, and autoimmunity. Defining the natural antibody repertoire is critical for identifying biomarkers, developing vaccines, controlling and preventing autoimmunity, and understanding the development and organization of the immune system. While natural antibodies to protein antigens have been studied in depth, little is known about natural antibodies to carbohydrate antigens. To address this, we profiled IgM from umbilical cord blood and matched maternal sera on a glycan microarray. Since standard methods to detect maternal contamination in cord serum did not have sufficient sensitivity for our study, we developed a highly sensitive microarray-based assay. Using this method, we found that over 50% of the cord samples had unacceptable levels of maternal contamination. For the cord samples with high purity, anti-glycan IgM antibodies were prevalent and recognized a broad range of non-human and human glycans. Using principal component analysis and hierarchical clustering, cord IgM repertoires showed a high degree of similarity with each other but were distinct from maternal IgM repertoires. Our results demonstrate...Continue Reading

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Citations

Dec 19, 2019·Viral Immunology·J Stewart NewJohn F Kearney
Dec 6, 2020·Annales d'endocrinologie·Nathalie GilliéronUrs E Nydegger
Apr 22, 2021·The Biochemical Journal·J Sebastian TemmeJeffrey C Gildersleeve
Jul 16, 2021·Frontiers in Immunology·Marta RibaCarme Pelegrí

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Methods Mentioned

BETA
glycosylation

Software Mentioned

GenePix
Excel
Partek Genomics Suite

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