Abstract
To investigate the anti-tumor activity of ursolic acid (UA) and its derivatives isolated from Aralia decaisneana on hepatocellular carcinoma both in vitro and in vivo. In vivo cytotoxicity was first screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, DNA ladder, flow cytometry analysis, Western blot and real time PCR were employed to elucidate the cytotoxic mechanism of UA. Implanted mouse hepatoma H22 was used to evaluate the growth inhibitory effect of UA in vivo. UA could significantly inhibit the proliferation of HepG2 and its drug-resistance strain, R-HepG2 cells, but had no inhibitory effect on primarily cultured normal mouse hepatocytes whereas all the six derivatives of UA could not inhibit the growth of all tested cell lines. Further study on mechanism demonstrated that apoptosis and G0/G1 arrest were involved in the cytotoxicity and cleavage of poly-(ADP-ribose)-polymerase (PARP). Downregulation of cyclooxygenase-2 (COX-2) protein and upregulation of heat shock protein (HSP) 105 mRNA correlated to the apoptosis of HepG2 cells treated with UA. In addition, UA also could inhibit the growth of H22 hepatoma in vivo. UA is a promising anti-tumor agent, but furth...Continue Reading
References
Dec 1, 1986·Cancer Letters·H TokudaY Ito
Oct 1, 1994·Gastroenterology·C E EberhartR N DuBois
Feb 1, 1994·The Journal of Clinical Investigation·R N DuBoisP R Bishop
Jan 1, 1994·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J LiuC D Klaassen
Mar 1, 1996·Phytochemistry·T MiyaseA Ueno
Jan 1, 1997·Cancer Letters·H Y HsuC C Lin
Jun 12, 1999·Planta medica·B S MinK Bae
Nov 21, 2000·Biological & Pharmaceutical Bulletin·A M DíazP Bermejo
Apr 12, 2001·Journal of Ethnopharmacology·D BaricevicA Zupancic
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 3, 2002·Journal of Biochemistry·Nobuyuki YamagishiTakumi Hatayama
Aug 16, 2002·European Journal of Biochemistry·Nobuyuki YamagishiTakumi Hatayama
Jul 11, 2003·Biochimica Et Biophysica Acta·Wen-Fang ChenMan-Sau Wong
Jul 23, 2003·Mini Reviews in Medicinal Chemistry·W N Setzer, M C Setzer
Sep 19, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·T J Raphael, G Kuttan
Oct 21, 2003·International Journal of Cancer. Journal International Du Cancer·Katharina M DetjenStefan Rosewicz
Oct 22, 2003·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·B BuecherH M Blottière
Dec 23, 2003·Cancer Immunology, Immunotherapy : CII·Chiara CastelliGiorgio Parmiani
Jan 28, 2004·Bioscience, Biotechnology, and Biochemistry·Norihiro BannoHoyoku Nishino
Jul 9, 2004·World Journal of Gastroenterology : WJG·Shu-Lan YuanJie Zhang
Jan 6, 2005·World Journal of Gastroenterology : WJG·Zhong-Zheng ZhuMeng-Chao Wu
Jul 12, 2005·Cancer Letters·Ze TianPeigen Xiao
Citations
Jan 2, 2008·BMC Cancer·Ze TianErxi Wu
Nov 28, 2013·BioMed Research International·El-Sayed S Abdel-HameedMahmood S Salman
Dec 31, 2010·Medical Oncology·Jing-song WangTao Xi
Mar 23, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Nighat Sultana
Nov 4, 2015·Journal of Asian Natural Products Research·Yan-Qiu MengMin-Jie Zhao
Apr 13, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Feng LiYanghao Guo
Jun 13, 2019·Oncology Letters·Xue-Min Feng, Xiu-Lan Su
Jan 29, 2009·Anti-cancer Drugs·Cong TangTao Xi
May 25, 2013·Nutrition and Cancer·Manjeshwar Shrinath BaligaPrincy L Palatty
Jun 24, 2017·Expert Opinion on Therapeutic Patents·Hidayat HussainIshtiaq Ahmed
May 3, 2012·Molecular Carcinogenesis·Wen-Ling ShihPing-Yuan Lin
Jan 1, 2014·Chinese Journal of Integrative Medicine·Li-li ZangZe-yao Tang
Nov 28, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Łukasz WoźniakKrystian Marszałek
Sep 29, 2020·Cancer Management and Research·Zixi ChenXiangdong Kang
Oct 5, 2011·World Journal of Hepatology·Roslin J Thoppil, Anupam Bishayee
Feb 13, 2021·Drug Design, Development and Therapy·Reyaz Hassan MirMubashir Hussain Masoodi
Apr 18, 2021·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Abedul HaqueArm R Amin
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Sávia C A LopesMônica C Oliveira