Anti-High Mobility Group Box 1 Neutralizing-Antibody Ameliorates Dextran Sodium Sulfate Colitis in Mice.

Frontiers in Immunology
Liping ChenFang Xiao

Abstract

High mobility group box 1 (HMGB1) is a ubiquitous nuclear protein in mammals. When released into the extracellular space, it acts as a damage-associated molecular pattern. This study investigates whether increased HMGB1 levels are found in the intestinal mucosa of ulcerative colitis (UC) patients, and whether an anti-HMGB1 neutralizing-antibody (HnAb) can inhibit the intestinal inflammation elicited by dextran sulfate sodium (DSS) in mice. Because toll-like receptor 4 (TLR4) is implicated in HMGB1-mediated immune cell activation, DSS colitis was also elicited in TLR4-deficient mice in the presence and absence of HnAb. The expression of HMGB1 in UC patients was examined. HnAb was administered via intraperitoneal injection to TLR4 deficient mice and their wild-type littermates, both being induced to colitis with DSS. Finally, the protective effect of HnAb and TLR4 deficiency were evaluated. In UC patients, HMGB1 was up-regulated in the inflamed colon. When administered during DSS application, HnAb alleviated the severity of colitis with a lower disease activity index, limited histological damages, and reduced production of proinflammatory cytokines. This antibody also limited colonic barrier loss, decreased colonic lamina propria...Continue Reading

References

Dec 9, 1998·Clinical and Experimental Immunology·L A DielemanE P Van Rees
Apr 14, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Masayuki FukataMaria T Abreu
Jun 30, 2007·Biochemical and Biophysical Research Communications·Shin MaedaMasao Omata
Jan 12, 2011·Annual Review of Immunology·Ulf Andersson, Kevin J Tracey
Jan 1, 2009·Molecular Medicine Reports·Hiroshi YamasakiMichio Sata
Jul 27, 2011·The American Journal of Gastroenterology·Roberta VitaliSalvatore Cucchiara
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Jul 11, 2014·Molecular Aspects of Medicine·Rui KangDaolin Tang
Feb 3, 2015·The Journal of Clinical Investigation·Xiaorong ZhuEugene B Chang
Nov 5, 2015·United European Gastroenterology Journal·Justin Côté-DaigneaultPierre Poitras
Apr 27, 2016·Journal of Basic Microbiology·Peris Mumbi MunyakaJean-Eric Ghia
May 14, 2016·Acta Pharmaceutica Sinica. B·Qingjie ChenWenjun Yin
Oct 26, 2016·Inflammatory Bowel Diseases·Matthew C ChoyPeter De Cruz
Apr 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yongfeng LiuJun Qin
Dec 22, 2017·Journal of Medicinal Chemistry·Sonya VanPatten, Yousef Al-Abed
Feb 1, 2018·Therapeutic Advances in Gastroenterology·Séverine VermeireAmy Marren
Mar 23, 2018·International Journal of Nanomedicine·Xuan-Xuan LiLiang Zhong
Aug 24, 2018·Journal of Crohn's & Colitis·Christian MaaserUNKNOWN European Crohn’s and Colitis Organisation [ECCO] and the European Society of Gastrointestinal and Abdominal Radiology [ESGAR
Oct 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zaopeng YangLi Tang
Mar 16, 2019·Frontiers in Microbiology·Lu XiaoJie Chen
Apr 19, 2019·International Journal of Molecular Sciences·Barbara RuderChristoph Becker
Jun 20, 2019·Current Drug Targets·Eyaldeva C VijayakumarKedar S Prabhavalkar
Jun 22, 2019·Current Gene Therapy·Jingjing ZhaoYufeng Liu
Jun 24, 2019·European Journal of Pharmacology·Yam Nath PaudelMohd Farooq Shaikh
Sep 21, 2019·European Review for Medical and Pharmacological Sciences·C-P WuL-Y Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
biopsies
Assay
Protein Assay
biopsy
scraping
transmission electron microscopy
FACS

Software Mentioned

GraphPad Prism
mothur
Tree Star
FlowJo
R Development Core
R
IPP

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier & Cytokines

Some cytokines are able to cross the blood brain barrier through transport systems and enter the cerebrospinal fluid and interstitial fluid spaces. Here is the latest research on cytokines crossing the blood brain barrier and how this can affect tissues within the CNS.