Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells.

Journal of Virology
Anna G DrannikKenneth L Rosenthal

Abstract

Cervicovaginal lavage fluid (CVL) is a natural source of anti-HIV-1 factors; however, molecular characterization of the anti-HIV-1 activity of CVL remains elusive. In this study, we confirmed that CVLs from HIV-1-resistant (HIV-R) compared to HIV-1-susceptible (HIV-S) commercial sex workers (CSWs) contain significantly larger amounts of serine antiprotease trappin-2 (Tr) and its processed form, elafin (E). We assessed anti-HIV-1 activity of CVLs of CSWs and recombinant E and Tr on genital epithelial cells (ECs) that possess (TZM-bl) or lack (HEC-1A) canonical HIV-1 receptors. Our results showed that immunodepletion of 30% of Tr/E from CVL accounted for up to 60% of total anti-HIV-1 activity of CVL. Knockdown of endogenous Tr/E in HEC-1A cells resulted in significantly increased shedding of infectious R5 and X4 HIV-1. Pretreatment of R5, but not X4 HIV-1, with either Tr or E led to inhibition of HIV-1 infection of TZM-bl cells. Interestingly, when either HIV-1 or cells lacking canonical HIV-1 receptors were pretreated with Tr or E, HIV-1 attachment and transcytosis were significantly reduced, and decreased attachment was not associated with altered expression of syndecan-1 or CXCR4. Determination of 50% inhibitory concentrations...Continue Reading

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Citations

May 3, 2013·Journal of Virology·Anna G DrannikKenneth L Rosenthal
Jan 11, 2013·Clinical & Developmental Immunology·Johanne PoudrierMichel Roger
May 29, 2012·The International Journal of Biochemistry & Cell Biology·Thomas VerrierIgnacio Garcia-Verdugo
May 9, 2014·American Journal of Reproductive Immunology : AJRI·Mickey V PatelCharles R Wira
Apr 18, 2013·BioResearch Open Access·Viraj J JasingheCarole Lavigne
Aug 11, 2016·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Wbeimar Aguilar-JimenezMaria T Rugeles
Mar 7, 2021·International Journal of Molecular Sciences·Ting SuYutaka Furutani
May 15, 2018·Journal of Reproductive Immunology·Mickey V PatelCharles R Wira

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