Anti-HIV compound assessment by two novel high capacity assays

Journal of Virological Methods
D R Averett

Abstract

Two novel, semi-automated assays for the assessment of compounds for activity against human immunodeficiency virus (HIV) are described. One assay uses quantitation of DNA by fluorescence to monitor reversal by test compounds of HIV-induced growth inhibition. The second assay measures the amount of HIV p24 by an indirect immunofluorescent technique. Both assays are sufficiently sensitive to allow multiple sampling of 96-well plates. Intra- and inter-assay variability were within acceptable limits. The two assays provide comparable results for given compounds. Retrovir (3'-azido, 3'-deoxythymidine, AZT) protected MT4 cells from HIV-induced growth inhibition, and inhibited the production of HIV p24. Consistent with the results of others, the anti-HIV potency of AZT was dependent on the concentration of the infecting virus. Interestingly, AZT-protected, HIV-infected MT4 cells grew faster than mock-infected MT4 cells, and inclusion of Interleukin 2 in the assay eliminated this effect.

References

Jun 1, 1987·Journal of Virological Methods·R PauwelsM Vandeputte
Aug 1, 1985·The Journal of Infectious Diseases·L S MartinS L Loskoski
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W E ReiherT K Brunck

❮ Previous
Next ❯

Citations

Jun 1, 1991·Journal of Virological Methods·R J GulakowskiM R Boyd
Mar 11, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Richard Hazen, E Randall Lanier
May 1, 1991·Antimicrobial Agents and Chemotherapy·D R AverettT A Krenitsky
Apr 1, 1994·Antimicrobial Agents and Chemotherapy·N A Van DraanenG W Koszalka
Sep 29, 2005·Antimicrobial Agents and Chemotherapy·Robert G FerrisLawrence R Boone
Jan 5, 2005·Nucleosides, Nucleotides & Nucleic Acids·Kristjan S GudmundssonChristopher McGuigan
Nov 12, 2003·Nucleosides, Nucleotides & Nucleic Acids·Kristjan S GudmundssonChristopher McGuigan
Jul 20, 2011·Journal of Women & Aging·Paige AverettCarol L Jenkins
Nov 27, 2001·Nucleosides, Nucleotides & Nucleic Acids·K S GudmundssonP L Feldman
Mar 21, 1998·Applied and Environmental Microbiology·C MaulluR Pompei
May 9, 2002·Chemical Reviews·Tanmaya Pathak
May 1, 1997·Antimicrobial Agents and Chemotherapy·S M DalugeT A Krenitsky
Jul 6, 2000·Journal of Medicinal Chemistry·K S GudmundssonL B Townsend
Nov 13, 2004·Journal of Medicinal Chemistry·Andrew L HopkinsDavid K Stammers
Jan 19, 1996·Journal of Medicinal Chemistry·N A Van DraanenG W Koszalka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.