PMID: 9430247Jan 16, 1998Paper

Anti-human immunodeficiency virus type 1 human monoclonal antibodies that bind discontinuous epitopes in the viral glycoproteins can identify mimotopes from recombinant phage peptide display libraries

AIDS Research and Human Retroviruses
L J BootsA J Conley

Abstract

A phage display library screening approach was used to identify peptide sequences that could bind to anti-HIV-1 MAbs whose binding specificities are complex. Most of the antibodies used recognize discontinuous epitopes in gp120 and one recognizes gp41. Both a 15-mer and a 21-mer display library (each with a complexity of greater than 60 x 10[6]) and two constrained, V3 region-biased libraries, all expressed as recombinant pIII protein of filamentous phage, were used. The unmapped anti-gp120 human MAb A32 recognized a set of related linear sequences and repeatedly identified a single phage sequence that could form a cyclic disulfide structure. Selection methods were also developed so that phage could be obtained by competition selection in the presence of antibody bound to native, monomeric gp120 antigen (used with MAb IgG1b12 and the anti-gp120 V3 region MAb 447-52D) or gp120 variable region 3 synthetic peptides (used with anti-gp120 V3 region MAb 19b). The potent, virus-neutralizing MAb IgG1b12 recognized numerous sequences and, when used in competition with gp120, recognized only one sequence. These studies extend the range of antibody determinant studies that can be performed with display phage libraries, demonstrate a worka...Continue Reading

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Citations

Jun 25, 1999·Journal of Virological Methods·K C GoughG C Whitelam
Nov 9, 2002·FEMS Immunology and Medical Microbiology·Foroogh NejatollahiJames P Burnie
Feb 24, 2011·Journal of the American Chemical Society·Joan G SchellingerJacquelyn Gervay-Hague
Jan 25, 2005·AIDS Research and Human Retroviruses·Karim DorghamGuy Gorochov
Jan 1, 2010·Journal of Virology·William L InceRonald Swanstrom
Feb 3, 2016·Frontiers in Bioengineering and Biotechnology·Andrea GroßJutta Eichler
Jan 26, 2011·PloS One·Holger ThieStefan Dübel
May 19, 2012·International Journal of Molecular Sciences·Sylvie DelhalleAndy Chevigné
Aug 3, 2017·Development·Gabriela EccoDidier Trono
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Victor Raul Gómez-RománGoar Gevorkian
Aug 31, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erez M BublilJonathan M Gershoni
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E O HochleitnerK B Tomer

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