Anti-hyaluronidase oligosaccharide derived from chondroitin sulfate a effectively reduces polyspermy during in vitro fertilization of porcine oocytes

Biology of Reproduction
H TatemotoTadashi Nakada

Abstract

The present study was conducted to examine the effects of chondroitin sulfate A-derived oligosaccharide (ChSAO) on hyaluronidase activity and in vitro fertilization (IVF) parameters. The activity of hyaluronidase extracted from preincubated boar sperm was completely blocked by ChSAO at concentrations of 10 microg/ml or higher. After in vitro maturation of porcine cumulus-oocyte complexes, some oocytes were freed from their cumulus cells, and cumulus-intact or cumulus-free oocytes were inseminated with sperm in IVF medium containing various concentrations of ChSAO (0.1-100 microg/ml). In cumulus-intact oocytes, the penetration and the polyspermy rates (39% and 28%, respectively) were significantly decreased by treatment with 100 microg/ml ChSAO compared with those of oocytes treated without ChSAO (63% and 52%, respectively). On the contrary, in cumulus-free oocytes, the addition of 10-100 microg/ml ChSAO significantly reduced the polyspermy rate compared with the control (25-30% versus 53%, respectively), whereas ChSAO had no effect on sperm penetration. Interestingly, ChSAO added to IVF medium significantly decreased the number of sperm bound to the zona pellucida (ZP) of cumulus-free oocytes in a concentration-dependent manner...Continue Reading

References

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Citations

Jul 3, 2009·The Journal of Reproduction and Development·Shogo YamauchiHideki Tatemoto
Jan 10, 2009·Animal Reproduction Science·Chi-Hun ParkChang-Kyu Lee
Dec 18, 2013·Acta Veterinaria Hungarica·Dora DapinoPatricia E Marini
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Feb 3, 2007·Zygote : the Biology of Gametes and Early Embryos·Hideki TatemotoTadashi Nakada
Mar 27, 2018·Reproduction in Domestic Animals = Zuchthygiene·B XuanY Jin

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Methods Mentioned

BETA
Assay
scraping
Fluorescence

Software Mentioned

Image PC
Statistical Analysis System R

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