Anti-idiotypic antibodies of a predefined specificity generated against CDR3VH synthetic peptides define a private anti-CD4 idiotype

Molecular Immunology
R AttanasioP Kanda

Abstract

A synthetic peptide corresponding to the third complementarity determining region (CDR) of the heavy chain (CDR3VH) of anti-Leu3a, a monoclonal anti-CD4 antibody which inhibits HIV gp120 binding to CD4, was used to elicit specific anti-peptide antibodies in rabbits. The anti-peptide antisera showed anti-idiotypic antibody (anti-Id) activity and recognized both the immunizing peptide and the intact cognate protein by ELISA. In addition, the antisera reacted with isolated heavy chains of anti-Leu3a by Western blot analysis. The lack of reactivity with a panel of monoclonal anti-CD4 antibodies suggested that the anti-peptide antisera recognize a private idiotype (Id) associated with the anti-Leu3a CDR3VH region. Further studies demonstrated the inability of the rabbit antisera to inhibit the binding of anti-Leu3a to the CD4 molecule. In addition, soluble recombinant CD4 was unable to inhibit the binding of the rabbit anti-peptide antisera to anti-Leu3a indicating that the CDR3VH region may not be involved in CD4 recognition. Anti-Id containing sera from mice, rabbits and nonhuman primates immunized with the intact anti-Leu3a molecule did not bind the CDR3VH synthetic peptide, suggesting that the corresponding region of anti-Leu3a ...Continue Reading

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Citations

Oct 1, 1991·Biologicals : Journal of the International Association of Biological Standardization·R AttanasioR C Kennedy
Jul 1, 1993·Journal of Neuroimmunology·C C MaierJ E Blalock
Jan 1, 1990·International Reviews of Immunology·R Attanasio, R C Kennedy
Jan 1, 2012·Pharmaceuticals·Ramachandran Murali, Mark I Greene
Sep 1, 1996·European Journal of Immunology·H LangeH Lemke
Feb 23, 2008·Critical Reviews in Clinical Laboratory Sciences·Sarah M R WilleWilly E E Lambert
Sep 17, 2004·International Reviews of Immunology·Sybille Muller

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