Anti-infective biomaterials with surface-decorated tachyplesin I

Biomaterials
Qiong XueJing Han

Abstract

Implants decorated with antimicrobial peptides (AMPs) can prevent infection and reduce the risk of creating antibiotic resistance. Yet the restricted mobility of surficial AMP often compromises its activity. Here, we report a simple but effective strategy to allow a more flexible display of AMP on the biomaterial surface and demonstrate its efficacy for wound healing. The AMP, tachyplesin I (Tac), is tagged with the polyhydroxyalkanoate-granule-associated protein (PhaP) and immobilized on haloarchaea-produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) via hydrophobic interaction. The PhaP-Tac coating effectively inhibits the growth of both Gram-negative and Gram-positive bacteria. It also increases the surface hydrophilicity to improve fibroblast proliferation in vitro, and accelerates wound healing by decreasing bacterial counts to below 105 CFU per gram of tissue in a deep-wound mouse model in vivo. Taken together, these findings demonstrate an effective strategy to realize the full potential of AMPs in imparting implants with an anti-microbial activity that is localized and potent.

Citations

Jan 16, 2019·Applied Microbiology and Biotechnology·Akhilesh Kumar SinghSatarudra Prakash Singh
Oct 13, 2019·Current Pharmaceutical Design·Uzma ArifInn-Kyu Kang
Apr 19, 2020·Applied and Environmental Microbiology·Aranzazu MatoM Auxiliadora Prieto
Sep 12, 2020·Advanced Materials·Saber Amin YavariMichiel Croes
Apr 10, 2019·Polymers·Ariagna L Rivera-Briso, Ángel Serrano-Aroca
Sep 13, 2020·Applied Microbiology and Biotechnology·Junyu ChenJing Han
Nov 27, 2020·International Journal of Biological Macromolecules·Zongbao ZhouShunqing Tang
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung Hyuk ImSoo Hyun Kim
Jul 3, 2021·Nanomaterials·Francisco G BlancoM Auxiliadora Prieto
Sep 6, 2018·Biomacromolecules·Xue-Mei CheGuo-Qiang Chen
Dec 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Aranzazu MatoM Auxiliadora Prieto

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