Anti-Inflammatory Activity in Colon Models Is Derived from Δ9-Tetrahydrocannabinolic Acid That Interacts with Additional Compounds in Cannabis Extracts

Cannabis and Cannabinoid Research
Rameshprabu NallathambiHinanit Koltai

Abstract

Introduction: Inflammatory bowel diseases (IBDs) include Crohn's disease, and ulcerative colitis. Cannabis sativa preparations have beneficial effects for IBD patients. However, C. sativa extracts contain hundreds of compounds. Although there is much knowledge of the activity of different cannabinoids and their receptor agonists or antagonists, the cytotoxic and anti-inflammatory activity of whole C. sativa extracts has never been characterized in detail with in vitro and ex vivo colon models. Material and Methods: The anti-inflammatory activity of C. sativa extracts was studied on three lines of epithelial cells and on colon tissue. C. sativa flowers were extracted with ethanol, enzyme-linked immunosorbent assay was used to determine the level of interleukin-8 in colon cells and tissue biopsies, chemical analysis was performed using high-performance liquid chromatography, mass spectrometry and nuclear magnetic resonance and gene expression was determined by quantitative real-time PCR. Results: The anti-inflammatory activity of Cannabis extracts derives from D9-tetrahydrocannabinolic acid (THCA) present in fraction 7 (F7) of the extract. However, all fractions of C. sativa at a certain combination of concentrations have a signi...Continue Reading

References

May 1, 1977·The Journal of Pharmacy and Pharmacology·R N Smith, C G Vaughan
Aug 7, 1970·Science·R MechoulamY Grunfeld
Jun 1, 1994·Clinical and Experimental Immunology·K MitsuyamaK Tanikawa
Nov 5, 2002·Journal of Clinical Pharmacology·Raphael MechoulamRuth Gallily
Dec 25, 2002·European Journal of Pharmacology·Kenneth IhenetuClifford J Whelan
Jul 5, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·R Balfour Sartor
Sep 19, 2007·British Journal of Pharmacology·E RybergP J Greasley
Jan 18, 2008·World Journal of Gastroenterology : WJG·Andreas Sturm, Axel U Dignass
May 2, 2009·Inflammatory Bowel Diseases·Martin A StorrKeith A Sharkey
May 16, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Angelo A Izzo, Michael Camilleri
May 3, 2011·Biological & Pharmaceutical Bulletin·Lucia Renee RuhaakLars Bohlin
Jul 14, 2011·British Journal of Pharmacology·Ethan B Russo
May 3, 2012·Inflammatory Bowel Diseases·Anita AnnaháziRichárd Róka
Jul 21, 2012·Phytotherapy Research : PTR·Giuseppe EspositoLuca Steardo
May 8, 2013·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Timna NaftaliFred Meir Konikoff
Oct 1, 2013·International Journal of Colorectal Disease·Kaija-Leena KolhoErkki Savilahti
Dec 4, 2013·Inflammatory Bowel Diseases·Alice Y ChuangJohn H Kwon
Dec 21, 2013·Pharmacology·Rudolf Schicho, Martin Storr
Jan 1, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Barbara RomanoAngelo A Izzo
Apr 20, 2014·Journal of Crohn's & Colitis·Geert R D'HaensPaul Rutgeerts
Jan 21, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Valerio ChiurchiùMauro Maccarrone
Aug 1, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A StančićR Schicho
Feb 3, 2016·Journal of Natural Products·Oier Aizpurua-OlaizolaAresatz Usobiaga
Oct 21, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Barbara RomanoFrancesca Borrelli

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Citations

Feb 16, 2019·Expert Review of Clinical Pharmacology·Nienke HalbmeijerLissy De Ridder
Jan 4, 2020·Clinical and Translational Gastroenterology·Melanie KienzlRudolf Schicho
Dec 14, 2019·Journal of Epidemiology and Community Health·Dean EurichJason Dyck
Mar 28, 2020·United European Gastroenterology Journal·Shelly Tartakover MatalonTimna Naftali
Apr 26, 2020·International Journal of Molecular Sciences·Antonelly Cassio Alves de CarvalhoSandra M Barbalho
Dec 3, 2020·International Journal of Molecular Sciences·Fatema Yeasmin, Hyong Woo Choi
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dvora NamdarHinanit Koltai
Jun 3, 2021·Journal of Personalized Medicine·Cristian ScheauAna Caruntu
May 18, 2021·Cannabis and Cannabinoid Research·Frances R HenshawGenevieve Z Steiner
Nov 20, 2020·European Journal of Gastroenterology & Hepatology·Abhilash Perisetti, Hemant Goyal

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Methods Mentioned

BETA
biopsies
enzyme-linked immunosorbent assay
ELISA
nuclear magnetic resonance

Software Mentioned

GraphPad
JMP
GraphPad Prism

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