Anti-Inflammatory Activity of β-thymosin Peptide Derived from Pacific Oyster (Crassostrea gigas ) on NO and PGE₂ Production by Down-Regulating NF-κB in LPS-Induced RAW264.7 Macrophage Cells

Marine Drugs
Dukhyun HwangGun-Do Kim

Abstract

β-thymosin is known for having 43 amino acids, being water-soluble, having a light molecular weight and ubiquitous polypeptide. The biological activities of β-thymosin are diverse and include the promotion of wound healing, reduction of inflammation, differentiation of T cells and inhibition of apoptosis. Our previous studies showed that oyster β-thymosin originated from the mantle of the Pacific oyster, Crassostrea gigas and had antimicrobial activity. In this study, we investigated the anti-inflammatory effects of oyster β-thymosin in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells using human β-thymosin as a control. Oyster β-thymosin inhibited the nitric oxide (NO) production as much as human β-thymosin in LPS-induced RAW264.7 cells. It also showed that oyster β-thymosin suppressed the expression of prostaglandin E₂ (PGE₂), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, oyster β-thymosin reduced inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oyster β-thymosin also suppressed the nuclear translocation of phosphorylated nuclear factor-κB (NF-κB) and degradation of inhibitory κB (IκB) in LPS-induced RAW264.7 cells. Thes...Continue Reading

References

Nov 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·R M Clancy, S B Abramson
Jul 1, 1996·Journal of Leukocyte Biology·M J Sweet, D A Hume
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·B F McAdamG A FitzGerald
Jan 12, 2000·Oncogene·C S WilliamsR N DuBois
Dec 5, 2000·Current Opinion in Chemical Biology·J T Groves, C C Wang
Jan 3, 2001·The Journal of Clinical Investigation·P P Tak, G S Firestein
Oct 3, 2002·Nature Reviews. Immunology·Toby LawrenceDerek W Gilroy
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Riku KorhonenEeva Moilanen
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Jan 27, 2007·Experimental Eye Research·Gabriel SosneMichelle Kurpakus Wheater
Nov 22, 2007·Trends in Molecular Medicine·Taro Kawai, Shizuo Akira
May 6, 2009·Biochemical Pharmacology·Alexander H Sprague, Raouf A Khalil
Mar 23, 2010·Cell·Sergei I GrivennikovMichael Karin
Apr 22, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Emanuela Ricciotti, Garret A FitzGerald
Oct 2, 2012·Mediators of Inflammation·Alexandra MedeirosC Henrique Serezani
Jun 12, 2013·Molecular and Cellular Biochemistry·Shumin LvGeng Xu
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Jul 15, 2016·The International Journal of Neuroscience·Tara ShababGokula Mohan
Mar 7, 2018·Expert Opinion on Biological Therapy·Justin B BelskyDaniel C Morris
Jun 20, 2018·Veterinary World·L A AbdulkhaleqM N M Hezmee
Dec 7, 2018·International Journal of Molecular Sciences·Qingjie XueHuabao Xiong

❮ Previous
Next ❯

Citations

Jul 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yun-Da YaoHua Zhou
Oct 29, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Guan-Yu WangGow-Chin Yen
Sep 29, 2019·Journal of Ethnopharmacology·Marzia VasarriDonatella Degl'Innocenti
Dec 16, 2020·Marine Drugs·Vida ŠimatFatih Özogul
Dec 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yang FuXinwang Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
ELISA
nuclear translocation
enzyme-linked immunosorbent assay
electrophoresis

Software Mentioned

MultAlin
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here