Anti-Inflammatory Activity of Marine Ovothiol A in an In Vitro Model of Endothelial Dysfunction Induced by Hyperglycemia

Oxidative Medicine and Cellular Longevity
Immacolata CastellanoA Pandolfi

Abstract

Chronic hyperglycemia is associated with oxidative stress and vascular inflammation, both leading to endothelial dysfunction and cardiovascular disease that can be weakened by antioxidant/anti-inflammatory molecules in both healthy and diabetic subjects. Among natural molecules, ovothiol A, produced in sea urchin eggs to protect eggs/embryos from the oxidative burst at fertilization and during development, has been receiving increasing interest for its use as an antioxidant. Here, we evaluated the potential antioxidative/anti-inflammatory effect of purified ovothiol A in an in vitro cellular model of hyperglycemia-induced endothelial dysfunction employing human umbilical vein endothelial cells (HUVECs) from women affected by gestational diabetes (GD) and from healthy mothers. Ovothiol A was rapidly taken up by both cellular systems, resulting in increased glutathione values in GD-HUVECs, likely due to the formation of reduced ovothiol A. In tumor necrosis factor-α-stimulated cells, ovothiol A induced a downregulation of adhesion molecule expression and decrease in monocyte-HUVEC interaction. This was associated with a reduction in reactive oxygen and nitrogen species and an increase in nitric oxide bioavailability. These result...Continue Reading

References

Jun 23, 2001·Molecular and Biochemical Parasitology·M R Ariyanayagam, A H Fairlamb
Mar 11, 2008·Biochimica Et Biophysica Acta·Immacolata CastellanoEmmanuele De Vendittis
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Feb 27, 2016·Scientific Reports·Immacolata CastellanoAnna Palumbo
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Jul 29, 2017·Diabetes/metabolism Research and Reviews·Pamela Di TomoGloria Formoso

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Citations

Jul 28, 2018·Natural Product Reports·Immacolata Castellano, Florian P Seebeck
May 15, 2019·Metabolites·Vadim V YansholeYuri P Tsentalovich
Nov 28, 2019·Proceedings. Biological Sciences·Marco GerdolImmacolata Castellano
Jan 17, 2019·Oxidative Medicine and Cellular Longevity·Mariarita BrancaccioImmacolata Castellano
Sep 24, 2020·Marine Drugs·Alfonsina MilitoAnna Palumbo
Nov 7, 2019·Oxidative Medicine and Cellular Longevity·Cristian Cezar LoginŞoimiţa Suciu
Aug 4, 2019·The Journal of Biological Chemistry·Mariarita BrancaccioImmacolata Castellano
Oct 15, 2020·Scientific Reports·Yuri P TsentalovichIliya M Odud
Jan 15, 2020·Free Radical Biology & Medicine·Alfonsina MilitoAnna Palumbo
Jul 18, 2021·Genome Biology and Evolution·Mariarita BrancaccioImmacolata Castellano

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Methods Mentioned

BETA
ion exchange chromatography
Assay
flow cytometry
PMA

Software Mentioned

FlowJo

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