Anti-Inflammatory and Gastroprotective Roles of Rabdosia inflexa through Downregulation of Pro-Inflammatory Cytokines and MAPK/NF-κB Signaling Pathways

International Journal of Molecular Sciences
Md Rashedunnabi AkandaByung-Yong Park

Abstract

Globally, gastric ulcer is a vital health hazard for a human. Rabdosia inflexa (RI) has been used in traditional medicine for inflammatory diseases. The present study aimed to investigate the protective effect and related molecular mechanism of RI using lipopolysaccharide (LPS)-induced inflammation in RAW 246.7 cells and HCl/EtOH-induced gastric ulcer in mice. We applied 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), nitric oxide (NO), reactive oxygen species (ROS), histopathology, malondialdehyde (MDA), quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blot analyses to evaluate the protective role of RI. Study revealed that RI effectively attenuated LPS-promoted NO and ROS production in RAW 246.7 cells. In addition, RI mitigated gastric oxidative stress by inhibiting lipid peroxidation, elevating NO, and decreasing gastric inflammation. RI significantly halted elevated gene expression of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) in gastric tissue. Likewise, RI markedly attenuated the mitogen-activated protein kinases (...Continue Reading

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Citations

May 18, 2020·International Journal of Molecular Sciences·Thanh Q C NguyenKaeko Kamei
Oct 28, 2019·The American Journal of Chinese Medicine·Hwa-Jeong LeeYoung Ran Kim
Oct 2, 2019·International Journal of Molecular Sciences·Ritva Tikkanen, David J Nikolic-Paterson
Mar 19, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Jun ZhangBi-Wei Song
Nov 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Catarina SerafimLeônia Batista
Aug 10, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Bruno Oliveira de VerasPenha Patricia Cabral Ribeiro
Sep 3, 2021·Planta medica·Thaise BoeingArquimedes Gasparotto Junior
Oct 12, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yizi ZhangDongli Li

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Methods Mentioned

BETA
MDA
nuclear translocation
protein assay
electrophoresis

Software Mentioned

Graph Pad
Graph Pad Prism

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