Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus

Biological Research
Nguyen Phuong ThaoChau Van Minh

Abstract

In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 μg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S) 5α-cholestane-3β,4β,6α,7α,8β,15α,16β,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 μM), and for (25S) 5α-cholestane-3β,6α,8β,15α,16β,26-hexol (1) and (25S) 5α-cholestane-3β,6α,7α,8β,15α,16β,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and 1.02 ± 0.01 μM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. This is the first report of the anti-inflam...Continue Reading

References

Jan 1, 1992·Clinical Immunology and Immunopathology·T Hirano
Apr 1, 1991·The Journal of Surgical Research·T J FaheyG T Shires
Jan 1, 1989·Free Radical Biology & Medicine·L L Smith, B H Johnson
Jan 1, 1993·Proceedings of the Society for Experimental Biology and Medicine·Q ZhouF A Kummerow
Nov 1, 1995·Immunobiology·K AupeixP Bischoff
Apr 16, 1998·Cellular and Molecular Life Sciences : CMLS·M LailleC Debitus
Dec 20, 2002·Nature·Carl Nathan
Dec 29, 2005·Journal of Medicinal Food·M A Louis JeuneJ Brown
Dec 29, 2007·The Journal of Pathology·J R Bradley
Sep 1, 2009·Expert Opinion on Investigational Drugs·Changhai DingGraeme Jones
Apr 23, 2010·International Immunology·Tadamitsu Kishimoto
Apr 23, 2010·Chemical & Pharmaceutical Bulletin·Ke PanTomofumi Miyamoto
May 12, 2011·Chemistry & Biodiversity·Guang DongYonghong Liu
Dec 4, 2012·Bioorganic & Medicinal Chemistry Letters·Nguyen Phuong ThaoYoung Ho Kim
Aug 16, 2013·Marine Drugs·Nguyen Phuong ThaoYoung Ho Kim
Sep 21, 2013·Journal of Natural Products·Nguyen Phuong ThaoYoung Ho Kim

❮ Previous
Next ❯

Citations

Jan 25, 2018·Journal of Cosmetic Dermatology·Margit Lw JuhászEllen S Marmur
Feb 27, 2020·Journal of Cosmetic Dermatology·Julie K NguyenJared Jagdeo
Oct 1, 2020·Natural Product Research·Le Thi VienChau Van Minh
Jul 5, 2016·Chemistry & Biodiversity·Timofey V MalyarenkoValentin A Stonik
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.