Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells

Mediators of Inflammation
Junying LuFang Wang

Abstract

Dysregulated inflammation is increasingly considered as the main cause of many diseases on which NOD1/NF-κB pathway plays an important role. Columbianetin (CBT) is derived from the root of the Chinese herb Radix Angelicae Pubescentis for treating inflammatory diseases. Although the anti-inflammatory effect of CBT has been reported, its anti-inflammatory mechanism was poorly studied. In this study, we explored the anti-inflammatory pathway of CBT in lipopolysaccharide- (LPS-) stimulated human peripheral blood mononuclear cell (PBMC) model. Inflammatory cytokine production in culture supernatant was assessed using ELISA assay, and the possible anti-inflammatory pathway of CBT was screened using qPCR array and enrichment analysis with DAVID6.8. To further confirm the targeted pathway of CBT, we pretreated PBMC with the selective NOD1 inhibitor ML130 and then measured the protein levels of the pathway by Western blotting. The result showed that CBT effectively suppressed the expressions of TNF-α, IL-6, MCP-1, and IL-1β in a dose-dependent manner and significantly downregulated 19 out of 32 differentially expressed genes, most of which were involved in the NOD1/NF-κB pathway, and also showed that CBT remarkably inhibited LPS-induced...Continue Reading

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Citations

Feb 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chao ZhangFang Wang
May 11, 2019·PloS One·Michael E BregenzerGeeta Mehta
Mar 23, 2020·Photodiagnosis and Photodynamic Therapy·Ampika Phutim MangkhalthonTeerasak Damrongrungruang

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Methods Mentioned

BETA
protein
ELISA
density gradient centrifugation
enzyme-linked immunosorbent assay
PCR
chips

Software Mentioned

MATLAB
DAVID6

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