Anti-Inflammatory Effect of Ginsenoside Rh2 -Mix on Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cells

Journal of Medicinal Food
Delgerzul BaatarSeong Gu Hwang

Abstract

Ginsenoside Rh2, a protopanaxadiol saponin from ginseng, has been reported to have strong anti-inflammatory activity. However, the concentration of ginsenoside Rh2 is very low (>0.001%) in the total ginseng extracted, which is not enough for production despite its high pharmacological effects. Thus, in this study, we evaluated the anti-inflammatory effect of ginsenoside Rh2-mix (GRh2-mix) on lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells. From the high-performance liquid chromatography analysis, it was confirmed that the GRh2-mix was mainly composed of 20(S)-Rh2, 20(R)-Rh2, Rk2, and Rh3. The LPS-stimulated RAW 264.7 cells were treated with different concentrations of GRh2-mix (100, 200, 400, 500 μg/mL). The cell counting kit-8 assay showed that the GRh2-mix treatment increased cell proliferation in LPS-stimulated RAW 264.7 murine macrophage cells. The GRh2-mix inhibited nitric oxide production in a dose-dependent manner, suggesting an anti-inflammatory effect. Furthermore, reverse transcription polymerase chain reaction and Western blot results also indicated that the GRh2-mix suppressed inflammatory genes such as iNOS, TNF-α, COX-2, IL-1β, IL-6, and NF-κB. In summary, these results suggest that the GRh2-...Continue Reading

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Citations

Mar 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui WangMin Chen
Jun 2, 2018·BioMed Research International·Md Abul Kalam AzadJie Yin
Nov 28, 2018·International Journal of Molecular Sciences·Ye Eun Jeong, Mi-Young Lee
Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shaoxia YeZhimin Zhao
Feb 25, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Heeok HongSeong Gu Hwang
May 12, 2021·International Journal of Systematic and Evolutionary Microbiology·Muhammad Zubair SiddiqiWan-Taek Im

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Methods Mentioned

BETA
ELISA
electrophoresis
PCR

Software Mentioned

Autochro 3000
STAT
SAS
ImageJ

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