Anti-Inflammatory Effect of Sulforaphane on LPS-Activated Microglia Potentially through JNK/AP-1/NF-κB Inhibition and Nrf2/HO-1 Activation.

Cells
Lalita SubediSun Yeou Kim

Abstract

Sulforaphane (SFN), a potent nuclear factor erythroid 2-related factor 2 (Nrf2) activator, is present in the species of the Brassicaceae, especially in broccoli sprouts. In this study, the effects of SFN against microglial activation and inflammation, and the potential mechanisms involved, were analyzed. As mitogen-activated protein kinase (MAPK) signaling plays a key role in microglial activation and inflammation, we focused on the role of SFN in regulating the MAPK signaling regulation of the inflammatory and anti-inflammatory cascades in lipopolysaccharide (LPS)-activated microglia. The anti-inflammatory and immunomodulatory effects of SFN were explored by evaluating the expression and secretion of inflammatory proteins, cytokines, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and activator protein-1 (AP-1) under pre- and post-treatment conditions. Under the SFN pre- and post-treatment conditions, the MAPK phosphorylation levels were significantly reduced in both acutely and chronically activated microglial cells. SFN also reduced the c-Jun N-terminal kinase (JNK) phosphorylation levels, which subsequently reduced NF-κB and AP-1 signaling. As a result, the expression of the inflammatory mediators (i...Continue Reading

References

Jul 14, 1995·The Journal of Biological Chemistry·M Karin
Mar 5, 2002·Nature Medicine·Tzong-Shyuan Lee, Lee-Young Chau
Aug 27, 2005·The Journal of Biological Chemistry·Paula MonjeJ Silvio Gutkind
Oct 22, 2008·Oncogene·D N Dhanasekaran, E P Reddy
Jan 19, 2010·Biochimica Et Biophysica Acta·Eun Kyung Kim, Eui-Ju Choi
Sep 16, 2010·Free Radical Biology & Medicine·Simone ReuterBharat B Aggarwal
Apr 2, 2011·The Journal of Biological Chemistry·Claude A PiantadosiHagir B Suliman
Dec 21, 2012·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Hyunjin Vincent KimYoungSoo Kim
Aug 29, 2013·Oxidative Medicine and Cellular Longevity·Andrea TarozziPatrizia Hrelia
Nov 6, 2013·Journal of Medicinal Chemistry·Francesco Peri, Valentina Calabrese
Mar 22, 2014·Seminars in Immunology·Guadalupe Sabio, Roger J Davis
Sep 10, 2014·International Journal of Molecular Sciences·Rui ZhangLi An
Jul 17, 2015·Oxidative Medicine and Cellular Longevity·Alessandra MasciLuciana Mosca
Aug 14, 2015·Journal of Leukocyte Biology·Allison J GreaneyMahtab Moayeri
Nov 20, 2015·Oxidative Medicine and Cellular Longevity·Yang BaiYang Zheng
Feb 5, 2016·Journal of Molecular Neuroscience : MN·Jing Sun, Guangxian Nan
Feb 18, 2016·Oxidative Medicine and Cellular Longevity·Christine A HoughtonJeff S Coombes
Apr 4, 2016·Environmental Pollution·Jing MengShu Tao
Jun 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lalita SubediSun Yeou Kim
Nov 23, 2016·American Journal of Respiratory Cell and Molecular Biology·Irene M J EurlingsMieke A Dentener
Apr 26, 2017·International Journal of Molecular Medicine·Jing Sun, Guangxian Nan
Jul 4, 2017·International Journal of Molecular Sciences·Lalita SubediSun Yeou Kim
Oct 3, 2018·International Journal of Molecular Sciences·Anastasiia BohushAnna Filipek

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Citations

Jan 30, 2020·International Journal of Molecular Sciences·Lalita SubediSun Yeou Kim
Sep 15, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·H AsciN Hasseyid
Aug 13, 2020·Cell and Tissue Research·Jinjuan LvLi Kong
Sep 15, 2020·Journal of Alzheimer's Disease : JAD·Wei WangJianping Jia
Jul 28, 2020·International Journal of Molecular Sciences·Zhiquan ChenFelix Becker
Aug 28, 2020·Frontiers in Immunology·Samanta C FunesAlexis M Kalergis
Nov 17, 2020·Frontiers in Cellular Neuroscience·Shannon E GilchristSassan Hafizi
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kensuke MitsunariHideki Sakai
Mar 7, 2021·Nutrients·Nadia MazarakisPaul V Licciardi
Jul 11, 2019·Mini Reviews in Medicinal Chemistry·Zehra CalisAbdülkerim Kasim Baltaci
Apr 24, 2020·Respiratory Medicine Case Reports·Richard I HorowitzJames Bruzzese
Feb 8, 2020·Current Neuropharmacology·Shareen Singh, Thakur Gurjeet Singh
May 6, 2021·International Journal of Molecular Sciences·Lalita SubediSun-Yeou Kim
May 26, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jessica GasparelloRoberto Gambari
Jun 10, 2021·Oxidative Medicine and Cellular Longevity·Zhenling ZhangZhijun Duan
Aug 5, 2021·Current Research in Toxicology·Somesh BanerjeePartha Roy

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Methods Mentioned

BETA
electrophoresis
ELISA
ELISAs

Software Mentioned

GraphPad Prism
Image [UNK] 2D Elite

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