Anti-inflammatory effect of transforming growth factor-beta1 in myoblast transplantation

Transplantation
F MerlyJ P Tremblay

Abstract

The inflammatory reaction that occurs during the 5 days after transplantation led at 3 days to the death of 70% of injected myoblasts. Use of anti-inflammatory agents appeared to be a possible way to increase myoblast survival. The application of gene transfer techniques to cell transplantation offers the potential for the prevention of inflammatory reaction. In this study, transforming growth factor-beta1 (TGF-beta1) gene was introduced in myoblasts with a retroviral vector to permit the secretion of this anti-inflammatory cytokine. Survival of (1) infected myoblasts expressing TGF-beta1 or (2) normal myoblasts transplanted with genetically modified cloned myoblasts was compared with survival of normal myoblasts. Expression of TGF-beta1 by myoblasts or by cotransplanted cells decreased myoblast mortality after 3 days by roughly 20% (66.0+/-3.0% in control vs. 46.3+/-4.2% and 46.2+/-5.9%). The increase of myoblast survival by TGF-beta1 expression was correlated with a lower polymorphonuclear cell and macrophage infiltration in muscles compared with control. In addition, cytotoxicity of neutrophils against myoblasts was assayed in vitro. The oxidation of myoblasts by activated neutrophils was decreased after infection of the myo...Continue Reading

References

May 1, 1992·Muscle & Nerve·J HuardJ P Tremblay
Jul 1, 1991·European Journal of Immunology·M TurnerM Feldmann
Jul 6, 1990·Science·A M LeferM A Palladino
Mar 1, 1982·The Journal of Investigative Dermatology·P P BradleyG Rothstein
Sep 28, 1995·The New England Journal of Medicine·J R MendellR Rice
Aug 1, 1994·Human Gene Therapy·J HuardG Karpati
Feb 1, 1994·The Journal of Clinical Investigation·J HuardJ P Tremblay
Sep 1, 1993·Neuromuscular Disorders : NMD·J P TremblayM Tremblay
Jan 1, 1993·Scandinavian Journal of Immunology·A M MalyginT Timonen
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E RazD A Carson
Jun 1, 1996·Journal of Neuropathology and Experimental Neurology·I KinoshitaJ P Tremblay
Jul 1, 1996·Muscle & Nerve·Y FanM Grounds

❮ Previous
Next ❯

Citations

Feb 19, 2000·Microscopy Research and Technique·D Skuk, J P Tremblay
Jan 10, 2003·Cell Transplantation·Daniel SkukJacques P Tremblay
Oct 10, 2003·Journal of Neuropathology and Experimental Neurology·Daniel SkukJacques P Tremblay
Nov 8, 2003·Immunological Investigations·Jannette M DufourDwaine F Emerich
Dec 29, 2005·Experimental Biology and Medicine·Lei YeEugene K W Sim
Sep 12, 2012·Cell Transplantation·Catherine GérardJacques P Tremblay
May 31, 2017·American Journal of Reproductive Immunology : AJRI·Taiki SamejimaTomoyuki Fujii
Oct 22, 2003·Current Opinion in Rheumatology·Daniel Skuk, Jacques P Tremblay
Aug 9, 2011·Journal of Muscle Research and Cell Motility·Christopher R RathboneRonald E Allen
Sep 10, 1998·The Journal of Cell Biology·Z QuJ Huard
May 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ken SuzukiMagdi H Yacoub
Mar 26, 2004·Cell Transplantation·Jannette M DufourCraig R Halberstadt
Jun 5, 2015·PloS One·Yunyi KangGiovanni Paternostro
Aug 2, 2002·Clinical Orthopaedics and Related Research·W Douglas BiggarPeter N Ray
Jan 8, 2004·Xenotransplantation·Jannette M DufourGregory S Korbutt
Jan 29, 2000·Journal of Autoimmunity·G J Prud'homme, C A Piccirillo
May 23, 2001·Neuromuscular Disorders : NMD·E EspinosL Bernheim
Oct 11, 2005·Physical Medicine and Rehabilitation Clinics of North America·Baohong CaoJohnny Huard
May 2, 2000·The Journal of Thoracic and Cardiovascular Surgery·B N MoraG A Patterson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Related Papers

Advances in Experimental Medicine and Biology
F E StockdaleJ X DiMario
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
Jun-wei LiJin Lin
Methods in Cell Biology
T A Rando, H M Blau
© 2022 Meta ULC. All rights reserved