Anti-inflammatory effects of ivy leaves dry extract: influence on transcriptional activity of NFκB

Inflammopharmacology
J Schulte-MichelsSebastian Franken

Abstract

EA 575® is an ivy leaves dry extract (DER 5-7.5:1, 30% ethanol) used against diseases of the lower respiratory tract associated with productive cough. EA 575® improves symptoms associated with chronic inflammatory bronchial conditions. Compared to its bronchospasmolytic and secretolytic properties, the anti-inflammatory effects of EA 575® are mostly untried. Therefore, we addressed the question of whether the anti-inflammatory effect of EA 575® is due to an impact on the NFκB pathway. NFκB nuclear translocation was visualized by immunofluorescence in J774.2 as well as HEK293 cells. In the latter, a luciferase-based reporter was used to monitor NFκB transcriptional activity. Phosphorylation of RelA and its inhibitor IκB was measured by Western blot analysis. Additionally, changes in the stability of NFκB:IκB complex were shown by protein fragment complementation. Decreased transcriptional activity of NFκB under treatment with EA 575® was also shown for a human monocytic as well as a human lung epithelial cell line. EA 575® is able to inhibit NFκB transcriptional activity by partially inhibiting its translocation to the nucleus after stimulation with TNFα. Furthermore, phosphorylation of IκBα is reduced while phosphorylation of R...Continue Reading

References

Apr 25, 2003·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Laid SelloumChahra Boudoukha
Jul 2, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H SüleymanR Elias
Apr 20, 2004·Assay and Drug Development Technologies·Helen YuJohn K Westwick
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ivan MattioliM Lienhard Schmitz
Nov 2, 2006·Biological & Pharmaceutical Bulletin·Michel David dos SantosGlória Emília Petto de Souza
Mar 11, 2008·Molecular and Cellular Biochemistry·Nadia El-Guendy, Anthony P Sinai
Apr 15, 2008·Archives of Pharmacal Research·Hyun Pyo KimSam Sik Kang
Jan 7, 2010·Journal of Ethnopharmacology·Katarzyna KazłowskaGuo-Jane Tsai
Feb 4, 2012·Genes & Development·Matthew S Hayden, Sankar Ghosh
Mar 14, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Haitao ShiXiaoyan Guo
Oct 16, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Su Jung HwangKyu-Won Kim
Feb 24, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Janka Schulte-MichelsHanns Häberlein
Mar 22, 2016·Cells·Frank ChristianRuaidhrí J Carmody
Jul 2, 2016·Pharmacology & Therapeutics·Patrick D Mitchell, Paul M O'Byrne
Nov 8, 2016·Chest·Patrick D Mitchell, Paul M O'Byrne
Jan 20, 2017·Respiratory Research·Clara E Green, Alice M Turner

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Citations

Jul 20, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Fabio MeurerSebastian Franken

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Methods Mentioned

BETA
nuclear translocation
Assay
PCR
transfection
ubiquitination

Software Mentioned

GeneArt
Agilent Chemstation

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