Anti-inflammatory effects of several plant extracts on porcine alveolar macrophages in vitro

Journal of Animal Science
Y LiuJ E Pettigrew

Abstract

Certain plant extracts are bioactive substances of some foods or traditional herbs, known to possess antioxidant, antibacterial, and perhaps immunoregulatory effects. This study investigated the in vitro anti-inflammatory effects of 7 plant extracts (anethol, capsicum oleoresin, carvacrol, cinnamaldehyde, eugenol, garlicon, and turmeric oleoresin) on porcine alveolar macrophages collected from weaned pigs (n = 6 donor pigs) by bronchoalveolar lavage. The experimental design for this assay was a 2 [with or without 1 μg lipopolysaccharide (LPS)/mL] × 5 (5 different amounts of each plant extract) factorial arrangements in a randomized complete block design. The application of plant extracts were 0, 25, 50, 100, and 200 μg/mL, except for cinnamaldehyde and turmeric oleoresin, which were 0, 2.5, 5, 10, and 20 μg/mL. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to determine the number of live cells, Griess assay was applied to detect nitric oxide (NO) production, and ELISA was used to measure tumor necrosis factor-α (TNF-α), IL-1β, transforming growth factor-β (TGF-β), and IL-10 in the cell culture supernatants of macrophages. The LPS increased (P < 0.001) the secretion of TNF-α, IL-1β, and TG...Continue Reading

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Citations

May 25, 2013·Animal Health Research Reviews·Bruce S SealCyril G Gay
Aug 5, 2014·BioMed Research International·Mohammad Hossein BoskabadyAzam Alavinezhad
Jul 22, 2014·Phytotherapy Research : PTR·Azam Alavinezhad, Mohammad Hossein Boskabady
Jul 3, 2013·Veterinary Immunology and Immunopathology·Ingrid WaldschmidtMari-Capucine Dupuis
May 24, 2016·Journal of Agricultural and Food Chemistry·Renato Pérez-RosésSalvador Cañigueral
Nov 16, 2013·Archives of Animal Nutrition·Katrin StelterSven Dänicke
Jan 31, 2020·Journal of Animal Science·Andrew R WilliamsAlexandra Blanchard
Mar 9, 2019·Frontiers in Veterinary Science·Xia XiongYanhong Liu
Aug 1, 2018·Veterinary Research·Hyun LillehojCyril G Gay
Apr 21, 2016·Equine Veterinary Journal·A E KaragianniR S Pirie
Apr 10, 2017·Journal of Dairy Science·J OhA N Hristov
Aug 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhaohai WuPeng Ji
Feb 13, 2021·Animals : an Open Access Journal From MDPI·Miguel Rabelo-RuizJuan Manuel Peralta-Sánchez

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