Anti-inflammatory Mechanism of Geniposide: Inhibiting the Hyperpermeability of Fibroblast-Like Synoviocytes via the RhoA/p38MAPK/NF-κB/F-Actin Signal Pathway

Frontiers in Pharmacology
Ran DengJun Fu

Abstract

Geniposide (GE) is the extraction and purification of iridoid glycosides from theGardenia jasminoides Ellis, which is a promising anti-inflammatory drug, but its mechanism of actions on rheumatoid arthritis (RA) has not been clarified. This study investigated the molecular mechanism behind GE reduced the high permeability of fibroblast-like synoviocytes (FLSs) derived from SD rats with adjuvant arthritis (AA), with the aims of observing the action of GE in AA rats and exploring new therapeutic strategies for RA treatment. The CCK-8 method was used to detect FLSs proliferation. The pro-inflammatory cytokines levels and anti-inflammatory cytokines levels in FLSs were determined by ELISA kits. FLSs permeability assay was performed on Transwell. Immunofluorescence was used to assay the arrangement and morphology of F-actin. The expression of the key molecules related to FLSs permeability (RhoA, p-p38MAPK, NF-κB p-p65 and F-actin) was detected by western blotting. After treatment with lipopolysaccharide (LPS), the proliferation and the permeability of the cells increased significantly (allP< 0.05). The expression of RhoA, p-p38MAPK, NF-κB p-p65 and F-actin in FLSs was higher compared with the control group, and F-actin was redistrib...Continue Reading

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Citations

Sep 24, 2020·International Journal of Rheumatic Diseases·Jie LiJin-Qi Li
Sep 14, 2019·Phytotherapy Research : PTR·Xu-Zhao Li, Shuai-Nan Zhang
Aug 15, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Jian-Ying WangYing Yuan
Jan 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jinxin LiuLi Wang
Mar 12, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tobias SiefenAlf Lamprecht
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Shamulailatpam Shreedarshanee DeviRanjana Arya
May 18, 2018·Journal of Agricultural and Food Chemistry·Haiou PanHao Ying

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Methods Mentioned

BETA
ELISA
Assay
Protein Assay
electrophoresis

Software Mentioned

Image
SPSS

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