Anti-Inflammatory Peptide Attenuates Edema and Promotes BMP-2-Induced Bone Formation in Spine Fusion

Tissue Engineering. Part a
Juliane D GlaeserHyun W Bae

Abstract

Recombinant human bone morphogenic protein-2 (BMP-2)-loaded absorbable collagen sponges (ACS) have been successfully used to enhance bone formation and to induce spinal fusion in humans. However, side effects, such as soft tissue edema and inflammation, have been reported. NEMO binding domain peptide (NBD) inhibits activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a central regulator of immune response. In this study, we investigated NBD's potential to reduce BMP-2-induced soft tissue inflammation without affecting BMP-2-mediated spinal fusion in rat. For evaluation of soft tissue inflammation, ACS containing BMP-2, BMP-2+NBD, NBD, or ACS only were implanted into intramuscular paraspinal sites of 32 rats. At day 2 postsurgery, edema formation at the implant sites was assessed using magnetic resonance imaging. T2-weighted relaxation time (T2-RT) values were increased in the BMP-2 group compared with BMP-2+NBD, NBD, and ACS groups. No difference in T2-RT values was detected between BMP-2+NBD versus NBD and ACS controls. Postsacrifice, histological analysis of the implant-surrounding zones showed increased mononuclear cell infiltration in the BMP-2 group compared with BMP-2+NBD and controls. The...Continue Reading

References

May 16, 2000·The Journal of Biological Chemistry·R Hofer-WarbinekR de Martin
Jan 3, 2001·The Journal of Clinical Investigation·A S Baldwin
Oct 6, 2001·American Journal of Respiratory and Critical Care Medicine·S J WilsonS T Holgate
Jan 30, 2003·Journal of Leukocyte Biology·J Alastair GracieIain B McInnes
Nov 19, 2003·Advanced Drug Delivery Reviews·M GeigerW Friess
Mar 9, 2004·Postȩpy higieny i medycyny doświadczalnej·Marek Fol, Teresa Gościcka
Dec 1, 2006·Journal of Leukocyte Biology·Paula K ShiremanLinda M McManus
Feb 27, 2007·The Spine Journal : Official Journal of the North American Spine Society·Brian PerriNeel Anand
Apr 1, 2008·The Spine Journal : Official Journal of the North American Spine Society·Wellington K Hsu, Jeffrey C Wang
Jan 8, 2009·Molecular Pain·Yi-Tin YenChih-Cheng Chen
May 19, 2009·Nature Medicine·Jia ChangCun-Yu Wang
Jan 27, 2010·BMC Biology·Nicholas MatlukJoseph M Verdi
Aug 28, 2010·The Spine Journal : Official Journal of the North American Spine Society·Ryan D MuchowPaul A Anderson
May 11, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yu Fu WangHua-Ping Liang
Jul 7, 2011·The Spine Journal : Official Journal of the North American Spine Society·Eugene J CarrageeBradley K Weiner
Feb 22, 2013·The Journal of Bone and Joint Surgery. American Volume·Hyun W BaeRick B Delamarter
Jun 14, 2013·Tissue Engineering. Part a·Jia ShenChia Soo
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Oct 30, 2013·Nature Reviews. Rheumatology·Makarand V Risbud, Irving M Shapiro
Jul 17, 2014·Molecular Endocrinology·Shizu Hirata-TsuchiyaEijiro Jimi
Feb 10, 2016·Tissue Engineering. Part B, Reviews·Aaron W JamesChia Soo
May 25, 2016·PLoS Medicine·Lionel Rostaing, Paolo Malvezzi

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Citations

Jul 7, 2020·Journal of Biomaterials Applications·Juliane D GlaeserHyun W Bae
Nov 23, 2019·International Journal of Molecular Sciences·Carsten W SchlickeweiJohannes Keller
Dec 28, 2018·International Journal of Molecular Sciences·Gabriele RussowJohannes Keller
Apr 30, 2021·Materials Today. Bio·L AndréeS C G Leeuwenburgh

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Methods Mentioned

BETA
ELISA
PCR
Assay
X-ray

Software Mentioned

ScancoMedical MicroCT
MIPAV
SAS
Prism

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